| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,436,912 | G→A | 40.5% | A349V (GCT→GTT) | ada ← | fused DNA‑binding transcriptional dual regulator and O6‑methylguanine‑DNA methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,436,912 | 0 | G | A | 40.5% | 49.0 / 73.1 | 106 | A349V (GCT→GTT) | ada | fused DNA‑binding transcriptional dual regulator and O6‑methylguanine‑DNA methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (21/42); new base A (15/28); total (36/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
AGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCAC > minE/1436850‑1436978 | aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:926747/69‑1 (MQ=255)aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:1721146/69‑1 (MQ=255)aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:1564371/69‑1 (MQ=255)aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:1545036/69‑1 (MQ=255)aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:2402559/69‑1 (MQ=255)aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:896810/69‑1 (MQ=255) gTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc < 1:3388113/68‑1 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:564787/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:55286/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:1196742/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:1243607/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:2706456/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:874032/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg > 1:2340701/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgagg > 1:2465465/1‑67 (MQ=255) gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTCCAGCTTCgcgg > 1:2733808/1‑67 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:2606166/69‑1 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:1033566/69‑1 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:1727625/69‑1 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:20014/69‑1 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:895691/69‑1 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:841323/69‑1 (MQ=255) ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg < 1:704778/69‑1 (MQ=255) gTTCATCATCGGCAAACAGATCAAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1016837/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGTCGCAGCAGTTgcgc < 1:870839/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:2181022/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:2175309/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:2276826/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:2004253/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1455167/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:2498408/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:185261/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1843001/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:3240139/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1181093/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1740300/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:651227/69‑1 (MQ=255) gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:862711/69‑1 (MQ=255) ttCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1552266/68‑1 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:3332310/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:1187092/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:2963488/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:2847750/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:1430187/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:659904/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:2649034/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:1589204/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:1940009/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:1798929/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:1691606/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt > 1:2150854/1‑69 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCtt > 1:82820/1‑68 (MQ=255) cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCtt > 1:713474/1‑68 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:2179808/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:1555617/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:1803887/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:1766257/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:10510/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:1251102/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:2689315/51‑1 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGa > 1:114159/1‑68 (MQ=255) ggCAAACAGATCCAACATTACCTCTCCTCATTATCAGCTTCGCGGCgcagc < 1:129881/51‑1 (MQ=255) gCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:2707184/50‑1 (MQ=255) aGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc < 1:2733027/44‑1 (MQ=255) gATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc < 1:1710274/51‑1 (MQ=255) aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAAcag > 1:1079477/1‑66 (MQ=255) aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCg > 1:1912193/1‑68 (MQ=255) aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCg > 1:1665316/1‑68 (MQ=255) ctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAgtgt > 1:2626615/1‑69 (MQ=255) ctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAgtgt > 1:473381/1‑69 (MQ=255) cATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGc < 1:1441177/69‑1 (MQ=255) ttttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGcc > 1:2678139/1‑68 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:1139665/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:178785/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:745800/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:325783/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:1677659/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:1433136/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:918485/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:2306728/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:2245432/69‑1 (MQ=255) tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGAGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:2814447/69‑1 (MQ=255) tttCAGCTTCGCGGCGAAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt < 1:222325/69‑1 (MQ=255) ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATc > 1:2603524/1‑69 (MQ=255) ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATc > 1:1049419/1‑69 (MQ=255) ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATc > 1:786570/1‑69 (MQ=255) ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTCCCAt < 1:2473590/68‑1 (MQ=255) ttCAGCTTCGCAGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt > 1:1943986/1‑68 (MQ=255) cAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATca < 1:1679697/68‑1 (MQ=255) cAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATca < 1:1860944/68‑1 (MQ=255) aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:690634/68‑1 (MQ=255) aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:653114/68‑1 (MQ=255) aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:3248182/68‑1 (MQ=255) aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:2500107/68‑1 (MQ=255) aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:2499315/68‑1 (MQ=255) aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATca < 1:485847/67‑1 (MQ=255) gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:832049/67‑1 (MQ=255) gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:3258630/67‑1 (MQ=255) gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:3120743/67‑1 (MQ=255) gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac < 1:2116611/67‑1 (MQ=255) | AGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCAC > minE/1436850‑1436978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |