| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,441,372 | G→C | 33.1% | A111A (GCG→GCC) | rcsD → | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,441,372 | 0 | G | C | 33.1% | 81.5 / 61.6 | 82 | A111A (GCG→GCC) | rcsD | phosphotransfer intermediate protein in two‑component regulatory system with RcsBC |
| Reads supporting (aligned to +/- strand): ref base G (38/16); new base C (20/7); total (58/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
TTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATC > minE/1441307‑1441433 | ttACAAGAGACGCGCCTGAAACTGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCg > 1:913112/1‑66 (MQ=255)ttACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:904539/1‑68 (MQ=255) cgcCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGctct < 1:1109390/69‑1 (MQ=255) cgcCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGctct < 1:3106397/69‑1 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:2746050/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:749022/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:1625459/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:874236/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:1468767/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:2182283/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:2956798/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:725464/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:2388316/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:1182577/1‑68 (MQ=255) tGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCAc > 1:3296525/1‑68 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTg > 1:1078582/1‑57 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGctct > 1:3323290/1‑62 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGctc > 1:3105710/1‑61 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:459154/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:1049329/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:2848301/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:2540550/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:599467/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:662885/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:1200901/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:536072/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:1207148/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:2952901/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:2239618/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:1265389/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:404036/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:3080712/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:1649951/1‑63 (MQ=255) aaCAGGATGTCTACTATCTGGAAAAACCACGCCGCAAAAAGGAAGCCTTAAACTTTGGCtctc > 1:2459475/1‑63 (MQ=255) aaCAGGATGTCTACTATCT‑GAAAAACCACGCCGCAAAACGGAAGCCTTAATCTTTGGCtctc > 1:3134839/1‑62 (MQ=255) ggATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTc < 1:3287037/69‑1 (MQ=255) ggATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTc < 1:95848/69‑1 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:2976928/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:1357166/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:1562890/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:1744192/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:412405/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:280099/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:2202429/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGtt > 1:2332670/1‑36 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGc > 1:735831/1‑65 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGc > 1:3260549/1‑65 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGc > 1:458787/1‑65 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGc > 1:1106996/1‑65 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGc > 1:228872/1‑65 (MQ=255) ctaTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGc > 1:2703377/1‑65 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCTTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:3156836/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:106798/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:772725/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:737047/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:440697/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:2253488/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:2263915/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:1503137/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:1391378/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:2906499/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:2972483/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:2935254/67‑1 (MQ=255) aTCTGGAGAAACCACGCCGCAAAACCGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTga < 1:3014703/67‑1 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAATCGTTAATCttt > 1:995595/1‑39 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGct > 1:998559/1‑43 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGAt > 1:3368769/1‑68 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:2937240/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:357118/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:2494702/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:3413822/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:1498959/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:3171023/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:18795/1‑69 (MQ=255) cTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATg > 1:1687345/1‑69 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:600216/69‑1 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:1616031/69‑1 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:1735052/69‑1 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:679827/69‑1 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:3213316/69‑1 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:3020191/69‑1 (MQ=255) ccACGCCGCAAAACGGAAGCCTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAACgg < 1:3316027/69‑1 (MQ=255) cGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATc > 1:2437541/1‑69 (MQ=255) | TTACAAGAGACGCGCCTGAAACAGGATGTCTACTATCTGGAGAAACCACGCCGCAAAACGGAAGCGTTAATCTTTGGCTCTCACGACAACTCAACGCTTGAGATGACTCAGCGGATGTCCACTTATC > minE/1441307‑1441433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |