| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,444,406 | T→C | 34.5% | intergenic (+27/+173) | rcsB → / ← rcsC | DNA‑binding response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,444,406 | 0 | T | C | 34.5% | 62.7 / 45.9 | 81 | intergenic (+27/+173) | rcsB/rcsC | DNA‑binding response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base T (36/17); new base C (22/6); total (58/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC > minE/1444350‑1444468 | gTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTa < 1:986533/66‑1 (MQ=255)gTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTa < 1:2848618/66‑1 (MQ=255)gTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTa < 1:403321/66‑1 (MQ=255)gTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTa < 1:454940/66‑1 (MQ=255)gTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTa < 1:898595/66‑1 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:2147584/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:664206/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:2247491/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:226069/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:199965/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:2924986/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:1821220/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:382107/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:2789444/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:2636795/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:2586216/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:1501203/1‑53 (MQ=255) tAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTCAgt > 1:14595/1‑53 (MQ=255) aaGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCAttt > 1:330064/1‑68 (MQ=255) aaGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTg > 1:1526406/1‑69 (MQ=255) aaGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTg > 1:545999/1‑69 (MQ=255) aaGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTg > 1:621959/1‑69 (MQ=255) gATAAAGAGTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2597892/1‑69 (MQ=255) gATAAAGAGTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2601903/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:1046368/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2933300/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2944601/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2027446/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:1901866/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:436451/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:1720620/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:525597/1‑69 (MQ=255) gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:806417/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2441978/1‑67 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:3165062/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:3255908/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:361808/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:377829/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:461247/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2732643/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:2670922/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2612080/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2273227/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2226829/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:2076557/1‑69 (MQ=255) aTCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATgcggc > 1:1760216/1‑68 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:1904732/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:109368/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:798335/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:1771922/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:2036891/69‑1 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:3227628/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:299598/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:1190377/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:981841/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:2699582/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:333855/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:562383/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:1820579/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:3330116/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:3302397/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:325906/69‑1 (MQ=17) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1059327/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:854162/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:963078/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:3174937/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:74186/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1400349/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1404360/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1815596/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1923458/1‑48 (MQ=255) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCa > 1:3272209/1‑37 (MQ=255) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:1499699/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:1764188/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:1246244/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:2994096/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:2451674/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:187617/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:2530442/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCA‑GGATTGCCGGATGCGGCGTAAACGCCTTATcc > 1:2449126/1‑69 (MQ=14) cgcgTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:3260300/68‑1 (MQ=255) | GTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC > minE/1444350‑1444468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |