| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,444,430 | C→G | 21.4% | intergenic (+51/+149) | rcsB → / ← rcsC | DNA‑binding response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,444,430 | 0 | C | G | 21.4% | 134.2 / 28.1 | 70 | intergenic (+51/+149) | rcsB/rcsC | DNA‑binding response regulator in two‑component regulatory system with RcsC and YojN/hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base C (42/13); new base G (8/7); total (50/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
GATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTA > minE/1444368‑1444492 | gATAAAGAGTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2597892/1‑69 (MQ=255)gATAAAGAGTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2601903/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2944601/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2027446/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:1901866/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2933300/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:1720620/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:436451/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:806417/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:1046368/1‑69 (MQ=255)gATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:525597/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGc > 1:2441978/1‑67 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:377829/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2273227/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2226829/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:2076557/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2612080/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:2670922/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:2732643/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:461247/1‑69 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:3165062/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg < 1:3255908/69‑1 (MQ=255) tAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCg > 1:361808/1‑69 (MQ=255) aTCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATgcggc > 1:1760216/1‑68 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:1771922/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:109368/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:1904732/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:798335/69‑1 (MQ=255) cACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa < 1:2036891/69‑1 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:2699582/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:981841/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:299598/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:3227628/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTa > 1:1190377/1‑68 (MQ=255) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:325906/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:333855/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:1820579/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:562383/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:3302397/69‑1 (MQ=17) aCCTGTAGGCCAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:3330116/69‑1 (MQ=17) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1059327/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1404360/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:3174937/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:74186/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:854162/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1815596/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:963078/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1923458/1‑48 (MQ=255) cTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCg > 1:1400349/1‑48 (MQ=255) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:1246244/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:2451674/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:1499699/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:2994096/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:1764188/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:187617/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCACGGATTGCCGGATGCGGCGTAAACGCCTTATc > 1:2530442/1‑69 (MQ=14) cAGATAAGACGCGTCAGTGTCTTATCTGGCATTTGCA‑GGATTGCCGGATGCGGCGTAAACGCCTTATcc > 1:2449126/1‑69 (MQ=14) cgcgTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:3260300/68‑1 (MQ=255) tCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCatta > 1:431233/1‑69 (MQ=255) tCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCatta > 1:3414798/1‑69 (MQ=255) tCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCatta > 1:1542004/1‑69 (MQ=255) tCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCatta > 1:68104/1‑69 (MQ=255) tCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCatta > 1:1807058/1‑69 (MQ=255) ttATCTGGCATTTGCACGGATTGCCGGATGCGGCGTaa < 1:1024765/38‑1 (MQ=255) ttGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTa < 1:1015276/69‑1 (MQ=255) tGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGGCTACGATTCCCATTATTTCAACAAATTa > 1:1378062/1‑68 (MQ=255) tGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTa > 1:1116688/1‑68 (MQ=255) tGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTa > 1:163312/1‑68 (MQ=255) tGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTa > 1:2771460/1‑68 (MQ=255) tGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTa > 1:1882922/1‑68 (MQ=255) | GATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTTATCTGGCATTTGCACCGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGATTCCCATTATTTCAACAAATTA > minE/1444368‑1444492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |