| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,445,416 | A→G | 70.7% | D655D (GAT→GAC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,445,416 | 0 | A | G | 70.7% | 63.4 / 26.3 | 65 | D655D (GAT→GAC) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base A (13/6); new base G (20/25); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01 | |||||||||||
GTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAT > minE/1445375‑1445480 | gTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGAT‑GCCCCGTCCATCATGCTGATCAGttttt < 1:1092088/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:1662996/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:1985803/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:1311779/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:2413047/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:2485128/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:3057713/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:1012326/69‑1 (MQ=255) tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:2869081/69‑1 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:2725259/1‑51 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:303694/1‑51 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:2866454/1‑51 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:2918360/1‑51 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:3344465/1‑51 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:297285/1‑51 (MQ=255) aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCcat > 1:3367032/1‑51 (MQ=255) aTTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATGATGCTGATCAg < 1:3027949/50‑1 (MQ=255) cccGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGccaga > 1:1980579/1‑66 (MQ=255) cccGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGccaga > 1:1442445/1‑66 (MQ=255) cccGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGccaga > 1:2115537/1‑66 (MQ=255) tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCg > 1:1912097/1‑69 (MQ=255) tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCg > 1:3323047/1‑69 (MQ=255) tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCg > 1:3101644/1‑69 (MQ=255) cGAGATGTCGCCGTCCATCATGCTGATCAGttttt < 1:2091267/35‑1 (MQ=255) agaTGTCGCCGTCCATCATTCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:117279/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:2099521/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1214378/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:2798629/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1206609/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:122930/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1232976/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1167173/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:129129/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:3239913/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1493801/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1981432/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:433293/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1770541/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:3370533/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:3429381/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:3396730/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:947967/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:689303/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:3031890/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:447936/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:3258170/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2988392/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2572795/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2510476/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2184313/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2132491/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1256618/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:155698/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1940991/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:176970/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1658471/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1647124/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaag > 1:12253/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaag > 1:674549/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaa > 1:652851/1‑67 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:1998596/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2063475/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2486571/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:1229136/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2578417/69‑1 (MQ=255) | GTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAT > minE/1445375‑1445480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |