| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,445,467 | G→A | 52.6% | T638T (ACC→ACT) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,445,467 | 0 | G | A | 52.6% | 1.1 / 66.5 | 76 | T638T (ACC→ACT) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base G (8/28); new base A (20/20); total (28/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGC > minE/1445399‑1445527 | tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCg > 1:1912097/1‑69 (MQ=255)tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCg > 1:3101644/1‑69 (MQ=255)tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCg > 1:3323047/1‑69 (MQ=255) agaTGTCGCCGTCCATCATTCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:117279/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1981432/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:2099521/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1770541/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:2798629/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:3239913/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1493801/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:3370533/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:129129/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:3429381/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1232976/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:122930/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:433293/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1167173/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1206609/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1214378/60‑1 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:947967/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:3396730/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2132491/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2184313/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2510476/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2572795/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:689303/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:2988392/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:3031890/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:447936/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:3258170/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1658471/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1647124/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1940991/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:155698/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:176970/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaagt > 1:1256618/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaag > 1:12253/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaag > 1:674549/1‑67 (MQ=255) agaTGTCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGaa > 1:652851/1‑67 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:1229136/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2578417/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2486571/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2063475/69‑1 (MQ=255) agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:1998596/69‑1 (MQ=255) tCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGc > 1:2224455/1‑69 (MQ=255) gCCGTCCATCATGCTTATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:1759294/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:1029195/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:487795/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:749939/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:140988/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:756594/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:3223888/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:3209021/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:3010945/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:2154455/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:2983609/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:2337538/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:2351374/69‑1 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:1820746/69‑1 (MQ=255) gCCGTACATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTg < 1:440221/69‑1 (MQ=255) ccATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:1866771/57‑1 (MQ=255) catcatGCTTATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAAt < 1:2901253/56‑1 (MQ=255) catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGcc > 1:2677972/1‑69 (MQ=255) catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGcc > 1:1668550/1‑69 (MQ=255) catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCAGTcc < 1:1522312/47‑1 (MQ=255) cAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGt > 1:3371530/1‑52 (MQ=255) cAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGAAGTTACGCTGTACGcc < 1:1505801/57‑1 (MQ=39) cAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGAAGTTACGCTGTACGcc < 1:1228291/57‑1 (MQ=39) cAGTTTTTCACAAATCGCCAGACCCAGACCAGTCCCCTGGAAGTTACGCTGTACGcc < 1:1829841/57‑1 (MQ=39) acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAgg < 1:3180406/68‑1 (MQ=255) acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAgg < 1:807758/68‑1 (MQ=255) acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAgg < 1:1863530/68‑1 (MQ=255) acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAgg < 1:2150730/68‑1 (MQ=255) acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAgg < 1:2343380/68‑1 (MQ=255) acaAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAgg < 1:333780/68‑1 (MQ=255) cccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAgcgc > 1:656123/1‑69 (MQ=255) cccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAgcgc > 1:593817/1‑69 (MQ=255) cccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAgcgc > 1:3427397/1‑69 (MQ=255) cccagaccGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAgcgc > 1:1284972/1‑69 (MQ=255) | TCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGC > minE/1445399‑1445527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |