| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,446,895 | G→A | 45.4% | I162I (ATC→ATT) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,446,895 | 0 | G | A | 45.4% | 5.2 / 32.8 | 44 | I162I (ATC→ATT) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base G (12/12); new base A (3/17); total (15/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
TTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAT > minE/1446835‑1446959 | ttCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGt > 1:869043/1‑67 (MQ=255)ttCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTc > 1:890654/1‑69 (MQ=255)ttCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTc > 1:1138324/1‑69 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGGGTTCATGCAAAGc < 1:2814878/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:112575/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:765926/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:605103/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:3329220/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:3206344/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:3197643/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2940343/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2641809/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2639769/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2410612/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2241628/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:1468416/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:1042043/69‑1 (MQ=255) ccAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2142044/69‑1 (MQ=255) cAGTAGAGGTTACTGCCACTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:762645/68‑1 (MQ=255) gagGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCaa > 1:326980/1‑69 (MQ=255) gagGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCaa > 1:2028747/1‑69 (MQ=255) tACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCgg > 1:81750/1‑69 (MQ=255) ccacTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGCTTTTAGCGCGGTATcg > 1:257754/4‑69 (MQ=255) ccacTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGCTTTTAGCGCGGTATcg > 1:2967954/4‑69 (MQ=255) ccacTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGCTTTTAGCGCGGTATcg > 1:660573/4‑69 (MQ=255) acTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGc < 1:2367793/50‑1 (MQ=255) atcatcTTGTGGTGCATTTCGATATTTATTGATGCGTTCATTCAAAGCTTTCAACGCGGTATCGCGTTc < 1:387167/69‑1 (MQ=255) atcatcTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTc < 1:262971/69‑1 (MQ=255) atcatcTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTc < 1:1632747/69‑1 (MQ=255) atcTTGTGGTGCATTTCGATATTTATTGATGCGtt < 1:2132794/35‑1 (MQ=255) atcTTGTGGTGCATTTCGATATTTATTGATGCGtt < 1:2220185/35‑1 (MQ=255) ggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa < 1:1377598/67‑1 (MQ=255) ggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa < 1:679354/67‑1 (MQ=255) ggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa < 1:1544184/67‑1 (MQ=255) tttCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACg < 1:2265205/68‑1 (MQ=255) tttCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACg < 1:2217886/68‑1 (MQ=255) tCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGt < 1:453586/42‑1 (MQ=255) tCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGt < 1:898782/42‑1 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACTGAAAt > 1:2834587/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:164158/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:1783441/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:273225/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:971666/1‑69 (MQ=255) tattGATGCGTTCATGAAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:1458808/1‑69 (MQ=255) | TTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAT > minE/1446835‑1446959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |