| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,446,916 | C→T | 20.2% | L155L (TTG→TTA) ‡ | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,446,916 | 0 | C | T | 20.2% | 85.9 / 13.2 | 59 | L155L (TTG→TTA) ‡ | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base C (29/18); new base T (3/9); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGC > minE/1446850‑1446980 | gagGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCaa > 1:326980/1‑69 (MQ=255)gagGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCaa > 1:2028747/1‑69 (MQ=255) tACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCgg > 1:81750/1‑69 (MQ=255) ccacTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGCTTTTAGCGCGGTATcg > 1:660573/4‑69 (MQ=255) ccacTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGCTTTTAGCGCGGTATcg > 1:257754/4‑69 (MQ=255) ccacTATCATCTTGTGGTGCATTTCGATATTTATTAATGCGTTCATGCAAAGCTTTTAGCGCGGTATcg > 1:2967954/4‑69 (MQ=255) atcatcTTGTGGTGCATTTCGATATTTATTGATGCGTTCATTCAAAGCTTTCAACGCGGTATCGCGTTc < 1:387167/69‑1 (MQ=255) atcatcTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTc < 1:1632747/69‑1 (MQ=255) atcatcTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTc < 1:262971/69‑1 (MQ=255) ggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa < 1:679354/67‑1 (MQ=255) ggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa < 1:1377598/67‑1 (MQ=255) ggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa < 1:1544184/67‑1 (MQ=255) tttCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACg < 1:2217886/68‑1 (MQ=255) tttCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACg < 1:2265205/68‑1 (MQ=255) tCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGt < 1:453586/42‑1 (MQ=255) tCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGt < 1:898782/42‑1 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACTGAAAt > 1:2834587/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:273225/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:971666/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:164158/1‑69 (MQ=255) tattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:1783441/1‑69 (MQ=255) tattGATGCGTTCATGAAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt > 1:1458808/1‑69 (MQ=255) gCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCa < 1:469778/68‑1 (MQ=255) gCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCa < 1:2664995/68‑1 (MQ=255) gCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCa < 1:2586856/68‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:375211/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:402467/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:1808958/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:2098380/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:911026/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:1136186/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:1186051/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:2342544/69‑1 (MQ=255) cGTTCATGCAAAGCTTTTAGCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg < 1:2165350/69‑1 (MQ=255) cATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCa > 1:1886050/1‑67 (MQ=255) cAAAGCTTTCAAGGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:3250166/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCg > 1:2034187/1‑49 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:2195370/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:2097131/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:2925166/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:1209611/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:260632/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:2323391/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:3260769/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:1624132/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:3313343/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGa > 1:3398357/1‑50 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc > 1:1018556/1‑57 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc > 1:1552555/1‑57 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc > 1:3273707/1‑57 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc > 1:2848789/1‑57 (MQ=255) cAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc > 1:2801863/1‑57 (MQ=255) cAAAGATTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGa > 1:2136643/1‑46 (MQ=255) aGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc < 1:3015883/69‑1 (MQ=255) aGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc < 1:2629766/69‑1 (MQ=255) aGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc < 1:205354/69‑1 (MQ=255) aGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc < 1:2098497/69‑1 (MQ=255) aGCTTTCAACGCGGTATCCCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc < 1:1967251/69‑1 (MQ=255) cTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGc > 1:2152154/1‑69 (MQ=255) | GAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGC > minE/1446850‑1446980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |