| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,447,108 | G→A | 50.7% | A91A (GCC→GCT) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,447,108 | 0 | G | A | 50.7% | ‑5.6 / 62.8 | 69 | A91A (GCC→GCT) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base G (24/10); new base A (16/19); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAG > minE/1447046‑1447167 | aaCCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAAc > 1:1353289/1‑69 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGTAACAATCAGAGTCAGCa > 1:1435195/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:914588/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:2353234/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:2462702/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:2552663/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:2648383/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:3000207/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:3099771/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:3139961/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:3389063/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:1102557/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:476507/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:1276390/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:1104498/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:591522/1‑46 (MQ=255) agaACCTCGCCAGGTGTTACTCATTGCGGAACAATCAGAGTCAGCa > 1:1338076/1‑46 (MQ=255) gaACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGcaca < 1:1135378/69‑1 (MQ=255) gtgtTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGtt > 1:2286443/1‑69 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:3060690/1‑50 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:2049580/1‑50 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:2476187/1‑50 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:2670086/1‑50 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:3109511/1‑50 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:372388/1‑50 (MQ=255) ttACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtct > 1:1640377/1‑69 (MQ=255) aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTtctc > 1:1304028/1‑68 (MQ=255) aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg > 1:2969626/1‑69 (MQ=255) aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg > 1:105578/1‑69 (MQ=255) aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg > 1:2558580/1‑69 (MQ=255) aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg > 1:2662038/1‑69 (MQ=255) aCTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCg > 1:3377700/1‑69 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:2571120/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:1043567/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:2209797/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:1287694/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:1495072/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:1730008/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:1778702/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:1937929/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:2007526/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:2087898/68‑1 (MQ=255) aCTCATTGCGGAACAATCAGAGACAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTtctc < 1:2966387/68‑1 (MQ=255) tCATTGCGGAACAATCAGAGTCAGCAAACAGCGGTTCAAACGCAGGAACATCCGTCTGCGTTtctc < 1:1394483/66‑1 (MQ=255) gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAcgc > 1:936754/1‑69 (MQ=255) gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAcgc > 1:3422171/1‑69 (MQ=255) gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAcgc > 1:1714166/1‑69 (MQ=255) gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAcgc > 1:2865695/1‑69 (MQ=255) gCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAcgc > 1:510145/1‑69 (MQ=255) gAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCAc > 1:2254146/1‑64 (MQ=255) gAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg < 1:172697/69‑1 (MQ=255) gAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCggg < 1:3259934/69‑1 (MQ=255) gAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCgg > 1:1821227/1‑68 (MQ=255) gAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCgg > 1:424191/1‑68 (MQ=255) gAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGAggg < 1:1028301/69‑1 (MQ=255) cAATCGGAGTCGGCAAACAGCGGTTCAAACGCAgg > 1:54277/1‑35 (MQ=255) aTCGGAGTCGTCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:1894969/69‑1 (MQ=255) aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:1407785/69‑1 (MQ=255) aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:1390964/69‑1 (MQ=255) aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:674552/69‑1 (MQ=255) aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:373632/69‑1 (MQ=255) aTCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:876906/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCTGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:2648891/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:1114141/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:3183933/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:1389268/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:2533046/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:1681163/69‑1 (MQ=255) aTCAGAGTCAGCAAACAGCGGTTCAAACGCAGGCACATCCGTCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:2637172/69‑1 (MQ=255) ggCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAg < 1:2874284/60‑1 (MQ=255) | AACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAG > minE/1447046‑1447167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |