| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,447,784 | G→A | 38.2% | D64N (GAT→AAT) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,447,784 | 0 | G | A | 38.2% | 32.5 / 36.5 | 60 | D64N (GAT→AAT) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base G (8/29); new base A (7/16); total (15/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01 | |||||||||||
CAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACT > minE/1447716‑1447848 | cagcagTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCg > 1:3425345/1‑69 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTTGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:1399983/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:2055609/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:2551978/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:2779483/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:1806693/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:2478782/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:3351607/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:3354451/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:353731/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:551666/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:1238489/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:2388758/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:216429/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:756819/69‑1 (MQ=255) tCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:889205/69‑1 (MQ=255) cTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCGCTAGGCAATCGCt < 1:3314625/68‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:2144073/66‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:2104064/66‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:2184144/66‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:2710889/66‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:1722125/66‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:1182749/66‑1 (MQ=255) aaaGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:673257/66‑1 (MQ=255) aatcTTTCTGCCGTTGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGa < 1:650007/62‑1 (MQ=255) aCTTTCTGCCGTCGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTAcc < 1:89210/69‑1 (MQ=255) cAACCTGTTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:1147749/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:2179066/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:1003270/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:226009/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:360951/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:3388715/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:3199605/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:2694463/68‑1 (MQ=255) cAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:3130437/68‑1 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGAtctc < 1:2413016/39‑1 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTAc > 1:2197810/1‑42 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:781600/67‑1 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:160689/67‑1 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:1019848/1‑69 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:1224480/1‑69 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:1935666/1‑69 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:1709733/1‑69 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:2416100/1‑69 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:2142192/1‑69 (MQ=255) aaCCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATc > 1:1313675/1‑69 (MQ=255) cAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTaaa < 1:1262685/68‑1 (MQ=255) cACTAGGCGGTCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAg < 1:2148414/69‑1 (MQ=255) cACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAg < 1:1625605/69‑1 (MQ=255) cACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAg < 1:369691/69‑1 (MQ=255) cACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAg < 1:1821092/69‑1 (MQ=255) cACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAg < 1:2051576/69‑1 (MQ=255) cACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAg < 1:3079644/69‑1 (MQ=255) tAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTa < 1:858987/48‑1 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:2201940/1‑69 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:2745736/1‑69 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:1030651/1‑69 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:386561/1‑69 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:3277908/1‑69 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:3076629/1‑69 (MQ=255) aGGCAATCGCTACGATCTCTACATCGACTTACCGCGTGAGGAGCGTATCCGCGCATTAAATGCAGAACt > 1:1916203/1‑69 (MQ=255) | CAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAGGCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCATTAAATGCAGAACT > minE/1447716‑1447848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |