| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,448,248 | T→A | 14.3% | I218I (ATT→ATA) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,448,248 | 0 | T | A | 14.3% | 142.9 / 15.1 | 70 | I218I (ATT→ATA) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base T (4/56); new base A (8/2); total (12/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
CTGGTTTGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACA > minE/1448187‑1448307 | cTGGTTTGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATatc < 1:501281/68‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTTAGCGCCAATATTGATATCATCAcc < 1:3056315/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2047827/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1078370/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2066239/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2070206/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2081870/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2404310/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2547504/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2550903/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2677587/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:2855656/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:288388/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:3009196/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:3199837/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:323847/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:348389/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:432925/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:443172/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:456677/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:490487/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:824919/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:837645/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:893952/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1369866/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1162209/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1775380/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:171497/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1099548/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1718755/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1792071/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1741841/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:183793/69‑1 (MQ=255) ttGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:1056823/69‑1 (MQ=255) ttGCTGATAACCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCAcc < 1:216650/69‑1 (MQ=255) tGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCg > 1:660879/1‑69 (MQ=255) tAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGcc > 1:1727461/1‑69 (MQ=255) tAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGcc > 1:987345/1‑69 (MQ=255) aaGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCct > 1:186791/1‑69 (MQ=255) ccTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCtctc < 1:1174622/69‑1 (MQ=255) ccTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCtctc < 1:1338965/69‑1 (MQ=255) ccTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCtctc < 1:2121501/69‑1 (MQ=255) tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACTAAatat < 1:3573/69‑1 (MQ=255) tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAatat < 1:994182/69‑1 (MQ=255) tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAatat < 1:1421341/69‑1 (MQ=255) tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAatat < 1:2037492/69‑1 (MQ=255) tttCTCCCGTCGCCTGAGCGCCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAatat < 1:2203153/69‑1 (MQ=255) tttCTCCCGTCGCCTGAGCGCCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAatat < 1:1306529/69‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:520123/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:934812/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:971081/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1235836/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2011218/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:339953/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2196333/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1714057/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:279997/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2704929/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2283136/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1824965/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2571729/68‑1 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:767831/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:974897/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:2237367/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:2684811/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:445183/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:730101/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:696725/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcac > 1:763882/1‑68 (MQ=255) ccAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1545178/66‑1 (MQ=255) ttGATATCATCACCGATGGCATCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1993679/60‑1 (MQ=255) | CTGGTTTGCTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACA > minE/1448187‑1448307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |