| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,448,281 | A→T | 17.8% | A229A (GCA→GCT) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,448,281 | 0 | A | T | 17.8% | 107.9 / 16.1 | 56 | A229A (GCA→GCT) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base A (17/29); new base T (8/2); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAAT > minE/1448221‑1448347 | tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACTAAatat < 1:3573/69‑1 (MQ=255)tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAatat < 1:994182/69‑1 (MQ=255)tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAatat < 1:1421341/69‑1 (MQ=255)tttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAatat < 1:2037492/69‑1 (MQ=255)tttCTCCCGTCGCCTGAGCGCCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAatat < 1:1306529/69‑1 (MQ=255)tttCTCCCGTCGCCTGAGCGCCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAatat < 1:2203153/69‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:279997/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2704929/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:339953/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2571729/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:520123/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2283136/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2196333/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2011218/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1235836/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1824965/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:934812/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:971081/68‑1 (MQ=255) cgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1714057/68‑1 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:730101/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:974897/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:445183/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:696725/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:2684811/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:2237367/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcaca > 1:767831/1‑69 (MQ=255) cgcCAATATAGATATCATCACCGATGGCCTCTCGACTCTGGCTCAAAATATTCCCACTCGATTACcac > 1:763882/1‑68 (MQ=255) ccAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1545178/66‑1 (MQ=255) ttGATATCATCACCGATGGCATCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1993679/60‑1 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:2412882/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:1021587/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:2780503/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:3108870/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:2601468/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:2333370/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:2282686/1‑69 (MQ=255) catcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAAt > 1:1933271/1‑69 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGAt > 1:1116861/1‑46 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2293486/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:11771/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1563963/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:1668017/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:218371/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:3023794/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:2562798/52‑1 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATg > 1:2428181/1‑69 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATg > 1:2608756/1‑69 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATg > 1:812446/1‑69 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATg > 1:1734525/1‑69 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATg > 1:965302/1‑69 (MQ=255) atcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATg > 1:3302153/1‑69 (MQ=255) atcaCCGATGGCCTATCGACTCTGGCACAAAATATTCCCACTCGATTACcac < 1:748186/52‑1 (MQ=255) ctctCGCCTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAAtca > 1:237137/1‑67 (MQ=255) ctctCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAAtca > 1:2013014/1‑67 (MQ=255) ctctGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCagag < 1:2423672/67‑1 (MQ=255) ctctGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCagag < 1:3224788/67‑1 (MQ=255) acaAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTtaat < 1:2644382/67‑1 (MQ=255) | TTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAAT > minE/1448221‑1448347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |