| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,448,347 | T→C | 57.7% | N251N (AAT→AAC) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,448,347 | 0 | T | C | 57.7% | 19.1 / 41.5 | 59 | N251N (AAT→AAC) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base T (18/7); new base C (31/3); total (49/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAT > minE/1448281‑1448413 | acaAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTtaat < 1:2644382/67‑1 (MQ=255) ttCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGccc > 1:1071615/1‑69 (MQ=255) ttCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGccc > 1:1964210/1‑69 (MQ=255) ttCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGccc > 1:132493/1‑69 (MQ=255) ttCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGccc > 1:1913620/1‑69 (MQ=255) ttCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGccc > 1:1613648/1‑69 (MQ=255) ttCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGccc > 1:757033/1‑69 (MQ=255) cACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAgg < 1:459247/68‑1 (MQ=255) cGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTg > 1:2891184/1‑69 (MQ=255) cGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTg > 1:841066/1‑69 (MQ=255) ttACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCG‑‑CAGGCACTGCGTGa < 1:1201894/69‑1 (MQ=255) aCCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCa > 1:3171416/1‑57 (MQ=255) aCCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGa > 1:273607/1‑69 (MQ=255) aCCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGa > 1:1898287/1‑69 (MQ=255) aCCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGa < 1:1674084/69‑1 (MQ=255) aCCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAAACTCGCCCAGGCACTGCGTGa < 1:885086/69‑1 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACACCCTCGCCCAGGCACTGCGTGaaa > 1:1691417/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2425752/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2751452/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2762573/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:97651/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2338966/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:3174459/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:3236188/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:3253043/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:337410/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:3421384/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:356984/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:36153/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:483760/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:787397/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:937889/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2298869/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:1394348/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:1532119/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:1578880/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2234753/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:1748342/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:1775545/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2156096/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:1971302/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2326862/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2065066/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGAACTGCGTGaaa > 1:197394/1‑67 (MQ=255) caATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCAGGGCACTGCGTGaaa > 1:2813756/1‑67 (MQ=255) caATTGCACGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:2153437/1‑67 (MQ=255) caATTGACCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAACAACCTCGCCCAGGCACTGCGTGaaa > 1:732771/1‑67 (MQ=255) aTTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAAcgcg < 1:3069404/69‑1 (MQ=255) aTTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAAcgcg < 1:3391857/69‑1 (MQ=255) tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGattatt > 1:126348/1‑68 (MQ=255) tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGattatt > 1:2353722/1‑68 (MQ=255) tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGattatt > 1:1570017/1‑68 (MQ=255) tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGattatt > 1:3347163/1‑68 (MQ=255) tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGattatt > 1:2055576/1‑68 (MQ=255) tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGattatt > 1:2083573/1‑68 (MQ=255) agtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGaa < 1:2198469/69‑1 (MQ=255) gtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGaa < 1:3013698/68‑1 (MQ=255) agagTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACg > 1:2269244/1‑69 (MQ=255) agTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGc < 1:2390923/68‑1 (MQ=255) agTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGc < 1:1298154/68‑1 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:184759/1‑69 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:2212942/1‑69 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:372220/1‑69 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:1566827/1‑69 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:79961/1‑69 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:1325453/1‑69 (MQ=255) aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAt > 1:3060589/1‑69 (MQ=255) | ACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGAT > minE/1448281‑1448413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |