| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,449,165 | A→G | 44.2% | Q524R (CAA→CGA) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,449,165 | 0 | A | G | 44.2% | 24.4 / 93.3 | 113 | Q524R (CAA→CGA) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base A (30/33); new base G (13/37); total (43/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-02 | |||||||||||
Only 100 of 130 total aligned reads displayed.
CTACTGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTG > minE/1449104‑1449227 | ctactGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTg > 1:1153204/1‑68 (MQ=255) actTAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:2904150/1‑60 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:2173607/1‑60 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa < 1:505401/60‑1 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:2408954/1‑60 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:2858956/1‑60 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa < 1:1946323/60‑1 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa < 1:1610935/60‑1 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa < 1:1712866/60‑1 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:257513/1‑60 (MQ=255) actGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:2551468/1‑60 (MQ=255) actGAATATCCTGATCAATGACGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCa > 1:101861/1‑60 (MQ=255) gAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:761387/67‑1 (MQ=255) gAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1495167/67‑1 (MQ=255) gAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1663734/67‑1 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:595410/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:1936701/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:195473/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2236183/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2083292/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2469579/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:59150/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:510310/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2796989/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:12699/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2372602/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:235193/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2342260/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2844645/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2941292/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2978200/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:3285189/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:3265581/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:102179/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1983580/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1963489/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2071841/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1071942/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:848917/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1448491/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1012612/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1194441/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGCCAGTat < 1:3077125/67‑2 (MQ=255) atCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGc < 1:1281718/69‑1 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGc > 1:55848/1‑68 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:1240268/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:84124/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:2378509/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:658808/1‑69 (MQ=255) cTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGAc < 1:2676514/69‑1 (MQ=255) cTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGAc < 1:184743/69‑1 (MQ=255) gATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACt < 1:2590189/68‑1 (MQ=255) cAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTcac > 1:943828/1‑68 (MQ=255) tGCCGTCCAGGCTATCAGTGCACGAGTGAAAATTCGTATTCGAACCTGGCAGTat < 1:1761224/55‑2 (MQ=25) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2886098/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2488233/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1134164/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1496253/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2403168/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:3103783/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:738711/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAg > 1:2420907/1‑68 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAg > 1:2135882/1‑68 (MQ=255) cTATCAGTGCACTAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:263251/69‑1 (MQ=25) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2618777/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:1232717/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2793104/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2784673/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2760562/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2646710/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2143471/69‑1 (MQ=37) cTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3004473/1‑67 (MQ=255) cACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACa < 1:650196/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:819696/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:759604/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:958232/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:321168/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:3197472/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:1425343/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:1553342/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:1744162/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:226194/69‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1232005/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:945743/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1312101/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1326208/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1457520/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:768781/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1766940/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2239099/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2399684/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2541212/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2679927/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3124956/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:345217/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3202375/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3254354/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTTGCATTg < 1:6435/64‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTg < 1:2630139/64‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGACTGTGGCATTg < 1:3420856/64‑1 (MQ=255) | CTACTGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTG > minE/1449104‑1449227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |