| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,449,175 | A→G | 50.0% | Q527Q (CAA→CAG) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,449,175 | 0 | A | G | 50.0% | ‑3.1 / 92.6 | 100 | Q527Q (CAA→CAG) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base A (29/21); new base G (13/37); total (42/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTG > minE/1449109‑1449227 | gAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1663734/67‑1 (MQ=255)gAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1495167/67‑1 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2469579/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:3152604/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:1936701/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:510310/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:59150/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:595410/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2083292/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:12699/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2796989/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:2590608/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTata > 1:1110414/1‑66 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2941292/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2978200/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2120408/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2071841/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1983580/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2342260/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:235193/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:2372602/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1963489/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1957157/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1012612/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:3265581/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:3250917/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:102179/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1071942/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:848917/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1194441/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1230833/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:687068/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:611154/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:1448491/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTat < 1:3285189/67‑2 (MQ=255) tatCCTGATCAATGCCGTCCAGGCTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGCCAGTat < 1:3077125/67‑2 (MQ=255) atCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGc < 1:1281718/69‑1 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:658808/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:1240268/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:137343/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:84124/1‑69 (MQ=255) tCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCg > 1:2378509/1‑69 (MQ=255) cTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGAc < 1:2676514/69‑1 (MQ=255) cTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGAc < 1:184743/69‑1 (MQ=255) gATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACt < 1:3293919/68‑1 (MQ=255) gATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACt < 1:2590189/68‑1 (MQ=255) cAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTcac > 1:943828/1‑68 (MQ=255) tGCCGTCCAGGCTATCAGTGCACGAGTGAAAATTCGTATTCGAACCTGGCAGTat < 1:1761224/55‑2 (MQ=25) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2488233/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:3103783/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2886098/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2755652/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:738711/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:2403168/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCaa < 1:1134164/50‑1 (MQ=255) ccGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAg > 1:2135882/1‑68 (MQ=255) cTATCAGTGCACTAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:263251/69‑1 (MQ=25) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:882404/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:1753491/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2143471/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2198203/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:1232717/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2793104/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2784673/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2760562/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2618777/69‑1 (MQ=37) cTATCAGTGCACGAGGGAAAATTCGTATTCGAACCTGGCAGTATAGCGACTCGCAACAGGCCATTTCGa < 1:2646710/69‑1 (MQ=37) cTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3004473/1‑67 (MQ=255) cACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACa < 1:650196/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:819696/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:759604/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:1425343/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:1553342/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:1744162/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:958232/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:321168/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:3197472/69‑1 (MQ=255) aaTTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTgg < 1:226194/69‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1312101/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2530574/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:945743/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2541212/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2399684/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2313748/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1232005/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:768781/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2679927/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:2239099/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3254354/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:281868/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1326208/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:3124956/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1457520/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1923947/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:345217/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTc > 1:1766940/1‑38 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTTGCATTg < 1:6435/64‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTg < 1:790662/64‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTg < 1:2630139/64‑1 (MQ=255) cAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGACTGTGGCATTg < 1:3420856/64‑1 (MQ=255) | GAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAAATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAACGGCTGTGGCATTG > minE/1449109‑1449227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |