| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,450,200 | A→G | 43.3% | L261L (CTA→CTG) | atoC → | fused response regulator of ato opeon, in two‑component system with AtoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,450,200 | 0 | A | G | 43.3% | 26.7 / 82.2 | 104 | L261L (CTA→CTG) | atoC | fused response regulator of ato opeon, in two‑component system with AtoS |
| Reads supporting (aligned to +/- strand): ref base A (25/34); new base G (26/19); total (51/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ACTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATTGC > minE/1450135‑1450265 | aCTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACa > 1:3435191/1‑68 (MQ=255)aCTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACa > 1:1734185/1‑68 (MQ=255)aCTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACa > 1:1337969/1‑68 (MQ=255)aCTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACa > 1:1941243/1‑68 (MQ=255)aCTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACa > 1:657925/1‑68 (MQ=255) cgacgaAATTGGCGAAATGCCGCTGTTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2055339/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2790609/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:1808695/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:811498/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:575331/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:497006/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2737874/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2322474/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2873910/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2908188/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:2978434/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:3094802/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:3179195/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:3358287/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:173102/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:1354389/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:1420513/1‑68 (MQ=39) cgacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAt > 1:1516857/1‑68 (MQ=39) gacgaAATTGGGGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:2306005/1‑68 (MQ=39) gacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:713037/1‑68 (MQ=39) gacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:3201237/1‑68 (MQ=39) gacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:1288381/1‑68 (MQ=39) gacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:80111/1‑68 (MQ=39) gacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:1722745/1‑68 (MQ=39) gacgaAATTGGCGAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAAtt > 1:1398607/1‑68 (MQ=39) cgaAATTGGCGAAATGCCGCTGGTACTATAAACCAAATTACTACGCATTCTACAGGAACGGGAATTTg < 1:1770673/68‑1 (MQ=255) aaTTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACg < 1:2536322/69‑1 (MQ=255) aTTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACgg < 1:2101434/69‑1 (MQ=255) aTTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACgg < 1:258645/69‑1 (MQ=255) aTTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACgg < 1:2522916/69‑1 (MQ=255) aTTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACgg < 1:736194/69‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:1576882/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:880609/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:1770064/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:968904/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:2498925/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:245377/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:1820421/68‑1 (MQ=255) gAAATGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:1716491/68‑1 (MQ=255) aaaTGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:433910/67‑1 (MQ=255) aaaTGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:20985/67‑1 (MQ=255) aaTGCCGCTGGTACTGCAAGCCAAATTACTACGCATTCTGCAGGAACGGGAATTTGAACGGATTgg < 1:808940/66‑1 (MQ=255) ccGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCAt < 1:744592/69‑1 (MQ=255) ccGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCAt < 1:1659157/69‑1 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGAc > 1:2081799/1‑66 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCa > 1:1216601/1‑68 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCa > 1:3325253/1‑68 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCa > 1:347517/1‑68 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCa > 1:3018968/1‑68 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCa > 1:490052/1‑68 (MQ=255) actacAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCa > 1:641836/1‑68 (MQ=255) acGCATTCTGCAGGAACGGGAATTTGAACGTATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:2326716/68‑1 (MQ=255) acGCATTCTGCAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:1785665/68‑1 (MQ=255) acGCATTCTGCAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:2624433/68‑1 (MQ=255) acGCATTCTGCAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:3388690/68‑1 (MQ=255) acGCATTCTGCAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:3152491/68‑1 (MQ=255) acGCATTCTGCAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:211577/68‑1 (MQ=255) cGCATTCTGCAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGca < 1:1941095/67‑1 (MQ=255) gCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCAt < 1:144521/41‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:2694560/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc > 1:852015/1‑60 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc > 1:894236/1‑60 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:3400133/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:877027/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:516503/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:333065/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:2404063/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:2597695/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:2117092/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:2700747/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:1826940/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc > 1:1766007/1‑60 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:1650146/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:1340121/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:313082/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc > 1:3279852/1‑60 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGc < 1:3290130/60‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:125016/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:1924049/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:2364889/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:258294/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:735734/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:2440188/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:689626/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:2574530/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:3346561/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:615191/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:572332/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:2634902/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc > 1:463825/1‑68 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:45800/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:3093898/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATtgc < 1:42280/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCACAAAAGTTGATATCCGCATCATtgc < 1:3028364/68‑1 (MQ=255) cTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACAATAAAAGTTGATATCCGCATCATtg > 1:1229494/1‑67 (MQ=255) | ACTCTGCTCCTCGACGAAATTGGCGAAATGCCGCTGGTACTACAAGCCAAATTACTACGCATTCTACAGGAACGGGAATTTGAACGGATTGGCGGCCATCAGACCATAAAAGTTGATATCCGCATCATTGC > minE/1450135‑1450265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |