| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,456,246 | G→A | 100% | P368P (CCC→CCT) | yfaQ ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,456,246 | 0 | G | A | 82.8% | 31.7 / 0.5 | 23 | P368P (CCC→CCT) | yfaQ | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (0/19); total (2/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAG > minE/1456190‑1456295 | gTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAg > 1:1213129/1‑68 (MQ=255)gTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAg > 1:2472301/1‑68 (MQ=255) tCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGc < 1:2254583/68‑1 (MQ=255) tCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGc < 1:1909618/68‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:10837/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:982083/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:66887/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:646069/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:3405069/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:2786127/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:2644720/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:2185515/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:2177078/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:18720/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1831916/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1773017/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1664839/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1573261/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1468606/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1006890/69‑1 (MQ=255) gCTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAACAAAg < 1:3173866/69‑1 (MQ=255) ccTGTGTGGCGCTGCTGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:1608549/68‑1 (MQ=255) gctgctGTCAGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAGAAAg < 1:2120971/59‑1 (MQ=255) | GTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAG > minE/1456190‑1456295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |