| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,458,274 | C→A | 37.6% | pseudogene (3681/4605 nt) pseudogene (3681/4104 nt) |
yfaS ← yfaS ← |
ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,458,274 | 0 | C | A | 37.6% | 88.4 / 88.7 | 109 | pseudogene (3681/4605 nt) pseudogene (3681/4104 nt) | yfaS yfaS | ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (46/22); new base A (10/31); total (56/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CAACTGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCAGAGAAATCTTCAGTTTTAG > minE/1458208‑1458341 | cAACTGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCgg > 1:3084550/1‑69 (MQ=255)cAACTGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCgg > 1:922066/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGCCTCCGGTGTa > 1:516606/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGgtgt > 1:702639/1‑68 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:1822171/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:1769222/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:3251620/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:693208/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:3017284/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:976793/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:3041354/1‑69 (MQ=255) tGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:680049/1‑69 (MQ=255) gggcTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCC‑‑‑GGAGACTCCGGTGTATCg > 1:3330280/3‑68 (MQ=255) gcgcTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:1008737/1‑68 (MQ=255) gcgcTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:2423196/1‑68 (MQ=255) gcgcTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:2115267/1‑68 (MQ=255) gcgcTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:906371/1‑68 (MQ=255) gcgcTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTa > 1:895237/1‑68 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGa > 1:1800839/1‑68 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:2020860/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:2100267/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:353137/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:804844/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:1504682/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:2129613/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:660181/1‑69 (MQ=255) ttttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAg > 1:2950625/1‑69 (MQ=255) atttttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCACTGGAGATTCAGGTGTATCGAg > 1:2102130/2‑69 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgt < 1:768784/66‑2 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:3247437/66‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:1369298/66‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:1290162/66‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:88082/66‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:1609600/66‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:1201662/66‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGa < 1:2686919/67‑1 (MQ=255) ttCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGa < 1:1272016/67‑1 (MQ=255) ggTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGTCTATCATTGa < 1:3026057/68‑1 (MQ=255) ggTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat < 1:3337815/69‑1 (MQ=255) ggTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat < 1:1215919/69‑1 (MQ=255) ggTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGa < 1:3203978/68‑1 (MQ=255) aCGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATcgcg > 1:380303/1‑68 (MQ=255) aCGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATc > 1:3205901/1‑65 (MQ=255) aCGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCgg > 1:2379384/1‑69 (MQ=255) aCGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCgg > 1:989331/1‑69 (MQ=255) aCGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCgg > 1:1492893/1‑69 (MQ=255) aCGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCgg > 1:847138/1‑69 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGGTGTCATTg < 1:145210/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:3055445/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:1465286/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:3086875/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2999197/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2984757/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2974012/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:3120443/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:670823/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2953405/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2946980/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2923628/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2866065/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2696614/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2631263/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2515216/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2488394/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:964588/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:724519/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2209349/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:2174644/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:1965475/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:808820/59‑1 (MQ=255) cGTTTGCCACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCACGCTGTCATTg < 1:2048959/59‑1 (MQ=255) gTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:1406791/43‑1 (MQ=255) gTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAgg < 1:2150977/43‑1 (MQ=255) tttGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGgtg < 1:560441/69‑1 (MQ=255) tttGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGgtg < 1:211146/69‑1 (MQ=255) cACCGCATCTGCCAGTGGAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:1443781/52‑1 (MQ=255) tCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCaga > 1:2870418/1‑68 (MQ=255) tCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCaga > 1:1491751/1‑68 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGTTCAGGCTATCATTGATATCGCGGGTGTCTTCCTCAGAGaa < 1:1168176/65‑1 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCAttta > 1:2286665/1‑38 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:812747/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:2803802/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:1935126/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:1133193/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:1473270/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:1552927/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:408948/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:2073604/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:2129047/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:2312904/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:2967146/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:2973195/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGat > 1:3062875/1‑41 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGa > 1:2060392/1‑40 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGa > 1:1883419/1‑40 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCAGAGaa < 1:1901292/65‑1 (MQ=255) aGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCAGAGaa < 1:1599671/65‑1 (MQ=255) aGTCGAGACTCCGGTGTATCGAGGATCAGGCTAACATTGat > 1:1219453/1‑41 (MQ=255) ggAGATTCAGGTGTATCGAGGATCAGGCTGTCATTg < 1:1838782/36‑1 (MQ=25) ccGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCAGAGAAATCTTCAGTTTTAg > 1:3148353/1‑69 (MQ=255) | CAACTGCGCTTTTTTCAGCAACGTCATGGTTAAGACGTTTGCCACCGCATCTGCCAGTGGAGACTCCGGTGTATCGAGGATCAGGCTATCATTGATATCGCGGGTGTCTTCCTCAGAGAAATCTTCAGTTTTAG > minE/1458208‑1458341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |