| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,458,571 | A→G | 37.8% | pseudogene (3384/4605 nt) pseudogene (3384/4104 nt) |
yfaS ← yfaS ← |
ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,458,571 | 0 | A | G | 37.8% | 18.8 / 26.9 | 45 | pseudogene (3384/4605 nt) pseudogene (3384/4104 nt) | yfaS yfaS | ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base A (5/23); new base G (5/12); total (10/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
TTACGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAGACG > minE/1458507‑1458639 | ttACGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAt < 1:820295/69‑1 (MQ=255)ttACGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAt < 1:720630/69‑1 (MQ=255)ttACGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAt < 1:1640591/69‑1 (MQ=255) aCGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAtt > 1:8509/1‑68 (MQ=255) aCGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAtt > 1:2592881/1‑68 (MQ=255) aCGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAtt > 1:2432846/1‑68 (MQ=255) aCGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAtt > 1:892648/1‑68 (MQ=255) aCGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCAtt > 1:441563/1‑68 (MQ=255) ccGATCGGCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:267714/68‑1 (MQ=255) ccGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:1832888/68‑1 (MQ=255) ccGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:1874599/68‑1 (MQ=255) ccGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:1898754/68‑1 (MQ=255) ccGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:3370739/68‑1 (MQ=255) ccGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:564189/68‑1 (MQ=255) ccGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGcc < 1:560249/68‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:3134950/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2599775/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2603849/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2643459/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:897328/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2191595/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:3255560/69‑1 (MQ=255) tCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:1978303/69‑1 (MQ=255) cGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:1586199/68‑1 (MQ=255) cGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2093484/68‑1 (MQ=255) tggctggcCTGCCAGTCGGCGTACCATGCCTATGCCGTGAGGAAGGCGTCGCCATTGCCATCTTCAccc < 1:1112293/69‑1 (MQ=255) tggctggcCTGCCAGTCGGCGTACCATGCCCATGCCGTGAGGAAGGCGTCGCCATTGCCATCTTCAccc < 1:2487592/69‑1 (MQ=255) tggctggcCTGCCAGTCGGCGTACCATGCCCATGCCGTGAGGAAGGCGTCGCCATTGCCATCTTCAccc < 1:2352661/69‑1 (MQ=255) tggctggcCTGCCAGTCGGCGTACCATGCCCATGCCGTGAGGAAGGCGTCGCCATTGCCATCTTCAccc < 1:1810568/69‑1 (MQ=255) tggctggcCTGCCAGTCGGCGTACCATGCCCATGCCGTGAGGAAGGCGTCGCCATTGCCATCTTCAccc < 1:2565974/69‑1 (MQ=255) atgcccatgccTTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGcc < 1:691474/69‑1 (MQ=255) atgcccatgccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGcc < 1:1945024/69‑1 (MQ=255) atgcccatgccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGcc < 1:1637416/69‑1 (MQ=255) atgcccatgccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGcc < 1:1987051/69‑1 (MQ=255) atgcccatgccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGcc < 1:2869734/69‑1 (MQ=255) atgcccatgccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGcc < 1:2704278/69‑1 (MQ=255) atgcccatgccGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2796468/39‑1 (MQ=255) atgcccatgccGTAAGGAAGGCGTCACCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGc < 1:687114/68‑1 (MQ=255) atgcccataccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTACGGGcc < 1:1308139/69‑1 (MQ=255) tgcccatgccGTAAGGAAGGCGTCACCATTGCCATCtt < 1:2391691/38‑1 (MQ=255) gccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCgg > 1:2196791/1‑58 (MQ=255) gccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGc > 1:3434234/1‑60 (MQ=255) gccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGc > 1:522200/1‑60 (MQ=255) gccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGc > 1:1326567/1‑60 (MQ=255) gccGTGAGGAAGGCGTCGCCATTGCCATCTTCACCCCACCAGGTAAAGCGCACTCCGGGc > 1:1214899/1‑60 (MQ=255) aCCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAg > 1:2226407/1‑66 (MQ=255) aCCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAGACg > 1:922468/1‑69 (MQ=255) aCCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAGACg > 1:3085351/1‑69 (MQ=255) aCCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAGACg > 1:2273580/1‑69 (MQ=255) aCCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAGACg > 1:1133598/1‑69 (MQ=255) | TTACGCCGATCGCCTGGCTGGCCTGCCAGTCGGCGTACCATGCCCATGCCGTAAGGAAGGCGTCACCATTGCCATCTTCACCCCACCAGGTAAAGCGCGCTCCGGGCCCCGCCAGTTGCATCAGCCGCAGACG > minE/1458507‑1458639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |