| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,464,254 | T→C | 22.8% | intergenic (‑58/+91) | yfaA ← / ← gyrA | hypothetical protein/DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,464,254 | 0 | T | C | 22.8% | 74.7 / 17.3 | 61 | intergenic (‑58/+91) | yfaA/gyrA | hypothetical protein/DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base T (12/35); new base C (7/7); total (19/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01 | |||||||||||
CACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTC > minE/1464187‑1464321 | caccacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGt < 1:2719660/68‑1 (MQ=255)caccacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGt < 1:1799593/68‑1 (MQ=255) ccacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGc < 1:1143175/68‑1 (MQ=255) ccacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGc < 1:2981554/68‑1 (MQ=255) ccacTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGc < 1:1611902/68‑1 (MQ=255) acTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:1398596/69‑1 (MQ=255) acTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:2328078/69‑1 (MQ=255) cTCATTGCCACATTCCTTGTGTATAGCCATCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:3281895/68‑1 (MQ=255) cTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:1370398/68‑1 (MQ=255) cTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTATACCGTGCCCt < 1:1619997/68‑1 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:2169162/1‑66 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:1137956/1‑66 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:211451/66‑1 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:2091262/66‑1 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:3238830/1‑66 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:2751859/1‑66 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:1742676/1‑66 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:3429133/66‑1 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:535172/1‑66 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt < 1:579342/66‑1 (MQ=255) cATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCt > 1:968390/1‑66 (MQ=255) aTTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGc < 1:1677426/54‑1 (MQ=255) tataGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACtt > 1:2650131/1‑69 (MQ=255) tataGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACtt > 1:2071303/1‑69 (MQ=255) cagccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGc < 1:2892266/69‑1 (MQ=255) cagccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGc < 1:2562650/69‑1 (MQ=255) cagccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGc < 1:3121568/69‑1 (MQ=255) cagccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGc < 1:182401/69‑1 (MQ=255) cagccaTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGc < 1:1366566/69‑1 (MQ=255) cagccaTTTTTTACGGGCACAGCCAAACTTTAACGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGc < 1:3350315/69‑1 (MQ=255) aTTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGa > 1:1480186/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:836913/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:278296/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:373180/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:1666704/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:2161157/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:331009/1‑69 (MQ=255) ttttttACGGGCACAGCCAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACTTTACAGCCTGGAc > 1:3266599/1‑69 (MQ=255) tttACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACtt > 1:1341946/1‑68 (MQ=255) ttACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACttt < 1:153002/68‑1 (MQ=255) acaGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTttcaattca > 1:352058/1‑68 (MQ=255) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCGAGACtt < 1:1571428/41‑1 (MQ=38) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACtt < 1:1925792/41‑1 (MQ=255) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACtt < 1:3190985/41‑1 (MQ=255) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACtt < 1:186655/41‑1 (MQ=255) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACtt < 1:3350335/41‑1 (MQ=255) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACtt < 1:480197/41‑1 (MQ=255) ccAAACTTTACCGCGCCCTAATACGACAAAAGCCCAGACtt < 1:3364817/41‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:2551462/69‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:3018490/69‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:9659/69‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:2537180/69‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:55015/69‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:1612786/69‑1 (MQ=255) aCTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:1821179/69‑1 (MQ=255) cTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:3356454/68‑1 (MQ=255) cTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGga < 1:2989434/68‑1 (MQ=255) gTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:2924688/69‑1 (MQ=255) gTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:2510582/69‑1 (MQ=255) gTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:1710306/69‑1 (MQ=255) gTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:965154/69‑1 (MQ=255) gTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:3033637/69‑1 (MQ=255) gTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:3025165/69‑1 (MQ=255) tGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTc < 1:2994010/68‑1 (MQ=255) | CACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTTTCAATTCAAACAAGGGAGATAGCTC > minE/1464187‑1464321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |