| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,464,999 | A→G | 47.5% | T658T (ACT→ACC) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,464,999 | 0 | A | G | 47.5% | ‑1.8 / 32.1 | 40 | T658T (ACT→ACC) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base A (3/18); new base G (10/9); total (13/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCGTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGT > minE/1464935‑1465061 | cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:1913329/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:1070292/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:60900/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:2969692/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:1876844/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:1712061/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:1176603/69‑1 (MQ=255)cGCCTTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:601865/69‑1 (MQ=255) cTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACGGttt < 1:2444241/45‑1 (MQ=255) tACCGGCGGTACGCAGACGGTTGAACTCGGTTAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGcc > 1:2270858/1‑68 (MQ=255) tACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGcc > 1:984892/1‑68 (MQ=255) tACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGcc > 1:898475/1‑68 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:1689408/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:578045/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:369686/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:3351892/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:2245921/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:3072641/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAg < 1:1250272/69‑1 (MQ=255) gcggTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGAGGTCGCCATGAAg < 1:2284655/69‑1 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCa > 1:2715020/1‑61 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:2868687/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:2899928/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:637687/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:2985555/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:219729/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:755809/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:1395707/1‑69 (MQ=255) gTACGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGACt > 1:1116656/1‑69 (MQ=255) gTAAGCAGACGGTTGAACTCGGTGAGGACGGTTTTCTTCACAGTACCGTTAGCGGTCGCCATGAAGAc > 1:3052784/1‑68 (MQ=255) aCGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACttt < 1:2865075/69‑1 (MQ=255) aCGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACttt < 1:2635235/69‑1 (MQ=255) aCGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACttt < 1:2630176/69‑1 (MQ=255) aCGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACttt < 1:151345/69‑1 (MQ=255) gAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGt < 1:689657/69‑1 (MQ=255) gAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGt < 1:2023053/69‑1 (MQ=255) gAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGt < 1:858885/69‑1 (MQ=255) gAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGt < 1:1729975/69‑1 (MQ=255) gAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGt < 1:99244/69‑1 (MQ=255) aGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGt < 1:997902/68‑1 (MQ=255) | CGCCGTCAACCAGTTTGATCGCCACTTTACCGGCGGTACGCAGACGGTTGAACTCGGTGAGGACAGTTTTCTTCACGGTACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGT > minE/1464935‑1465061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |