| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,465,077 | A→G | 100% | T632T (ACT→ACC) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,465,077 | 0 | A | G | 88.2% | 43.2 / ‑1.8 | 25 | T632T (ACT→ACC) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (0/22); total (2/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAG > minE/1465013‑1465141 | tACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGTCACTGGCAGGATCGCAGTGa < 1:2745206/69‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1266213/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:769928/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:722002/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:3405847/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:3227209/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:3177638/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:3108882/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:3003055/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:2831769/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:2385053/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:2357837/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:2073191/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1994985/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1937682/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:191027/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1839252/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1783461/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1666418/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:1571690/68‑1 (MQ=255) tCACCGGCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:101819/68‑1 (MQ=255) tCACCGGCAGGATAGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:957105/68‑1 (MQ=255) accggCAGGATCGCGGTGATACGTTCGTCCTGTTCCAGCGGCAGCAGGTTGACGATCGGACGACcg < 1:636212/63‑1 (MQ=255) tCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAg > 1:538694/1‑69 (MQ=255) tCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAg > 1:744473/1‑69 (MQ=255) | TACCGTTAGCGGTCGCCATGAAGACTTTCACGCCTTCTTCAAACTCGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAG > minE/1465013‑1465141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |