Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,465,344 G→A 58.4% L543L (CTC→CTT gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,465,3440GA58.4% 7.5 / 23.6 36L543L (CTC→CTTgyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base G (13/2);  new base A (14/7);  total (27/9)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGC  >  minE/1465286‑1465406
                                                          |                                                              
cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:838344/69‑3 (MQ=255)
cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:703185/69‑3 (MQ=255)
cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:2960080/69‑3 (MQ=255)
cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:2761471/69‑3 (MQ=255)
cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:1658010/69‑3 (MQ=255)
cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:2656385/69‑3 (MQ=255)
         gcgcTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:2742175/1‑69 (MQ=255)
         gcgcTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCt                                              >  1:2120392/1‑68 (MQ=255)
                      aGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca                                                      <  1:452866/47‑3 (MQ=25)
                       gAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAg                                <  1:1942827/68‑1 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:1127620/1‑41 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:766112/1‑41 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:425209/1‑41 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:301651/1‑41 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:2445739/1‑41 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:220801/1‑41 (MQ=255)
                                     cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:1332637/1‑41 (MQ=255)
                                       tAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                             >  1:135419/1‑39 (MQ=255)
                                       tAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGAt                 >  1:2147542/1‑67 (MQ=255)
                                       tAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGt               >  1:3090489/1‑69 (MQ=255)
                                          cGTAGCTCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCt             >  1:3411022/1‑68 (MQ=255)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:1717004/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:904009/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:1026356/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:1155462/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:1176613/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:3360712/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:3173026/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:3165950/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:1687665/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:1691623/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:246312/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:171417/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:2374345/3‑46 (MQ=37)
                                             aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg                                >  1:2289261/3‑46 (MQ=37)
                                               cccTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAg                                <  1:1582545/44‑1 (MQ=255)
                                                      gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc  <  1:1428404/67‑1 (MQ=255)
                                                      gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc  <  1:1590804/67‑1 (MQ=255)
                                                      gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc  <  1:2510781/67‑1 (MQ=255)
                                                      gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc  <  1:2474367/67‑1 (MQ=255)
                                                      gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc  <  1:2470423/67‑1 (MQ=255)
                                                          |                                                              
CACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGC  >  minE/1465286‑1465406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: