| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,465,350 | C→G | 68.7% | V541V (GTG→GTC) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,465,350 | 0 | C | G | 68.7% | 72.8 / 46.1 | 67 | V541V (GTG→GTC) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base C (14/7); new base G (14/32); total (28/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
CACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTC > minE/1465286‑1465415 | cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:2960080/69‑3 (MQ=255)cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:2761471/69‑3 (MQ=255)cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:2656385/69‑3 (MQ=255)cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:838344/69‑3 (MQ=255)cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:703185/69‑3 (MQ=255)cACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:1658010/69‑3 (MQ=255) gcgcTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:2742175/1‑69 (MQ=255) gcgcTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCt > 1:2120392/1‑68 (MQ=255) aGAAAGCGGCTGATACTTCACGTAACCCTGGTGAGAAAGCGTGAcca < 1:452866/47‑3 (MQ=25) gAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAg < 1:1942827/68‑1 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:1127620/1‑41 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:301651/1‑41 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:2445739/1‑41 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:220801/1‑41 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:425209/1‑41 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:766112/1‑41 (MQ=255) cTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:1332637/1‑41 (MQ=255) tAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt > 1:135419/1‑39 (MQ=255) tAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGAt > 1:2147542/1‑67 (MQ=255) tAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGt > 1:3090489/1‑69 (MQ=255) cGTAGCTCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCt > 1:3411022/1‑68 (MQ=255) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:3360712/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:1176613/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:3165950/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:246312/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:904009/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:3173026/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:2374345/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:2289261/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:1026356/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:1155462/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:1717004/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:171417/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:1691623/3‑46 (MQ=37) aacccTGGTGAGAAAGCGTGACCACCACATCTTCCTGGGTGATCAg > 1:1687665/3‑46 (MQ=37) cccTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAg < 1:1582545/44‑1 (MQ=255) gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc < 1:1428404/67‑1 (MQ=255) gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc < 1:2470423/67‑1 (MQ=255) gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc < 1:2474367/67‑1 (MQ=255) gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc < 1:2510781/67‑1 (MQ=255) gagagaGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc < 1:1590804/67‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:999736/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:3067284/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:99502/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:3103525/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:418289/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:816588/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:506817/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1833405/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1174761/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1180030/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1389728/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1526386/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1538660/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1571969/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1598528/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1726917/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2993756/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:1857782/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2142959/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2175462/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2397433/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:24720/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2548296/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2736680/69‑1 (MQ=255) cGTGACCACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCGGCGCTGTTAGCGGTGATTTc < 1:2751647/69‑1 (MQ=255) cGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTc > 1:1329355/1‑69 (MQ=255) | CACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTC > minE/1465286‑1465415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |