| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,465,518 | G→A | 34.8% | I485I (ATC→ATT) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,465,518 | 0 | G | A | 34.8% | 63.0 / 47.3 | 86 | I485I (ATC→ATT) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base G (41/15); new base A (14/16); total (55/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 | |||||||||||
CTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTT > minE/1465458‑1465579 | ttctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:2340464/2‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:3323116/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:2460837/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:2158399/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:2485677/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:27643/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:2816511/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:323916/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:1749572/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:15160/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:1447484/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:900516/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:843404/1‑67 (MQ=255)ctctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACACTTCCGCGATc > 1:3384485/1‑64 (MQ=255) tctTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATcc < 1:586007/69‑1 (MQ=255) ggATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCcagcag < 1:407669/67‑1 (MQ=255) ggATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCcagcag < 1:3335733/67‑1 (MQ=255) ggATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCcagcag < 1:1800352/67‑1 (MQ=255) ggATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCcagcag < 1:2069829/67‑1 (MQ=255) ggATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCcagcag < 1:812059/67‑1 (MQ=255) gATCACTTCCATCAGACGATAGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCcagcag < 1:893059/66‑1 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:932043/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:436942/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:2437432/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:2452089/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:2455906/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:3203131/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:550021/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:461897/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:3269194/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:2523649/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:380815/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:1301135/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:2253559/1‑57 (MQ=255) aTCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCAATCTGa > 1:2264655/1‑57 (MQ=255) tCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGctct > 1:1631731/1‑68 (MQ=255) tCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCtt > 1:2463547/1‑69 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:1992305/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:1159180/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:1410484/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:1491632/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:433732/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:1699570/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:2945208/69‑1 (MQ=255) ttCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTa < 1:2184126/69‑1 (MQ=255) tCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATtcg > 1:2520590/1‑68 (MQ=255) cAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATtcg > 1:2093335/1‑67 (MQ=255) cAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATtcg > 1:2802163/1‑67 (MQ=255) cAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATtcg > 1:2837330/1‑67 (MQ=255) cAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATtcg > 1:2152409/1‑67 (MQ=255) cAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATtc > 1:665759/1‑66 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:257714/1‑45 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:3403245/1‑45 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:2166572/1‑45 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:1593451/1‑45 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:3163049/1‑45 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:3018383/1‑45 (MQ=255) aGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGa > 1:1221108/1‑45 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2254855/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2194297/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:1260433/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:420037/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:85955/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2334683/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2681146/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2050429/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:1700708/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2850819/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2035413/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:3165261/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:1123723/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:2963304/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:3093305/52‑1 (MQ=255) aaGAATACGCAACAGTTCCGCAATCTGATCCAGCAGCTCTTTGTATtcgtcg < 1:3101065/52‑1 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGa > 1:365010/1‑68 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:1350761/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:1169783/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:866750/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:669269/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:1308283/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:3431471/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:534578/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:2423863/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:1600293/1‑69 (MQ=255) tACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGAc > 1:3432675/1‑69 (MQ=255) tCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGtt > 1:1717515/1‑67 (MQ=255) | CTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTT > minE/1465458‑1465579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |