| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,465,692 | A→C | 22.0% | A427A (GCT→GCG) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,465,692 | 0 | A | C | 22.0% | 72.2 / 18.4 | 50 | A427A (GCT→GCG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base A (25/14); new base C (5/6); total (30/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGC > minE/1465626‑1465757 | gtagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTTGCCAg < 1:2854844/68‑1 (MQ=255)gtagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAg < 1:2382027/68‑1 (MQ=255)gtagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAg < 1:2299469/68‑1 (MQ=255)gtagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAg < 1:2030056/68‑1 (MQ=255)gtagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAg < 1:1688143/68‑1 (MQ=255)gtagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAg < 1:1970582/68‑1 (MQ=255) tagtaCAGACCATCACGCACGCCGATCTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAg > 1:115673/1‑67 (MQ=255) tagtaCAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCa < 1:1145440/69‑1 (MQ=255) cAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGt > 1:2910475/1‑67 (MQ=255) cAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGt > 1:130815/1‑67 (MQ=255) cAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGt > 1:2591491/1‑67 (MQ=255) cAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGt > 1:703152/1‑67 (MQ=255) cAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGt > 1:2365897/1‑67 (MQ=255) aCCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAg < 1:881344/69‑1 (MQ=255) aCCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAg < 1:2185198/69‑1 (MQ=255) ccATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGc < 1:862461/69‑1 (MQ=255) gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:678414/69‑1 (MQ=255) gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:179873/69‑1 (MQ=255) gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:2527330/69‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:1344327/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:2790928/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:2413676/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:3415502/66‑1 (MQ=255) ccAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:256772/65‑1 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:2351103/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:561290/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:3168276/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:2016210/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:3116026/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACgg > 1:487899/1‑66 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:392579/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2640429/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:3379064/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:615799/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:3099136/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2951788/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1099653/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2640952/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1319348/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:877380/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1565431/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1579344/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2211912/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1608364/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1991853/1‑69 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:239353/1‑68 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:1503507/1‑68 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:1057632/1‑68 (MQ=255) tcgtcgCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:1020575/42‑1 (MQ=37) gCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGc < 1:168240/69‑1 (MQ=255) | GTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGC > minE/1465626‑1465757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |