| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,465,701 | G→C | 26.8% | L424L (CTC→CTG) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,465,701 | 0 | G | C | 26.8% | 56.2 / 20.2 | 41 | L424L (CTC→CTG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base G (19/11); new base C (5/6); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCA > minE/1465635‑1465764 | aCCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAg < 1:881344/69‑1 (MQ=255)aCCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAg < 1:2185198/69‑1 (MQ=255) ccATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGc < 1:862461/69‑1 (MQ=255) gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:179873/69‑1 (MQ=255) gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:2527330/69‑1 (MQ=255) gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:678414/69‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:3415502/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:1344327/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:2790928/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:2413676/66‑1 (MQ=255) ccAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:256772/65‑1 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:3116026/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:561290/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:2016210/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:3168276/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:2351103/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACgg > 1:487899/1‑66 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2211912/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1991853/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:615799/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1608364/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1579344/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2640429/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2640952/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1565431/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2951788/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:3099136/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:877380/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1319348/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:3379064/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1099653/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:392579/1‑69 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:1057632/1‑68 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:239353/1‑68 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:1503507/1‑68 (MQ=255) tcgtcgCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:1020575/42‑1 (MQ=37) gCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGc < 1:168240/69‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:1792059/66‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:69815/66‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:1380744/66‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:928590/66‑1 (MQ=255) | ACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCA > minE/1465635‑1465764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |