| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,465,710 | G→A | 28.8% | A421A (GCC→GCT) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,465,710 | 0 | G | A | 28.8% | 36.2 / 12.9 | 38 | A421A (GCC→GCT) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base G (19/8); new base A (5/6); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATG > minE/1465647‑1465778 | gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:678414/69‑1 (MQ=255)gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:2527330/69‑1 (MQ=255)gcCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAAc < 1:179873/69‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:2790928/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:2413676/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:1344327/66‑1 (MQ=255) tCCAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:3415502/66‑1 (MQ=255) ccAGCCATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:256772/65‑1 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:3168276/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:2351103/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:2016210/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:3116026/1‑69 (MQ=255) ccATTCCGGACGCGCAGCATCGTCGCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGCTGCCACgg > 1:561290/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACgg > 1:487899/1‑66 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2211912/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:877380/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2640952/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2640429/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1099653/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:615799/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1319348/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:2951788/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1565431/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:3099136/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1991853/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:392579/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:3379064/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1608364/1‑69 (MQ=255) ttCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGAtt > 1:1579344/1‑69 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:1057632/1‑68 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:239353/1‑68 (MQ=255) ccGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTa > 1:1503507/1‑68 (MQ=255) tcgtcgCCCGCACGTTCCAGCATCGCAGCAACGTTGCCCAGc < 1:1020575/42‑1 (MQ=37) gCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGc < 1:168240/69‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:928590/66‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:1792059/66‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:69815/66‑1 (MQ=255) tCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCa < 1:1380744/66‑1 (MQ=255) gcgGCAACGTTGCCCCGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGgcgc > 1:1191270/1‑68 (MQ=255) gcgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGgcgc > 1:1447491/1‑68 (MQ=255) gcgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGgcgc > 1:2749520/1‑68 (MQ=255) gcgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGgcgc > 1:1472347/1‑68 (MQ=255) gcgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGgcgc > 1:2070528/1‑68 (MQ=255) gcgGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGACGgcgc > 1:141848/1‑68 (MQ=255) ggCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCAt < 1:2692725/68‑1 (MQ=255) ggCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATg < 1:1232845/69‑1 (MQ=255) ggCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATg < 1:3225824/69‑1 (MQ=255) ggCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATg < 1:717111/69‑1 (MQ=255) | GCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATG > minE/1465647‑1465778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |