Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,469,421 A→G 61.2% G767G (GGT→GGC)  yfaL ← adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,469,4210AG61.2% 59.5 / 70.3 108G767G (GGT→GGC) yfaLadhesin
Reads supporting (aligned to +/- strand):  ref base A (31/11);  new base G (24/42);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 1.73e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TTGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAGGC  >  minE/1469359‑1469468
                                                              |                                               
ttGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCg                                           >  1:1837104/1‑69 (MQ=255)
ttGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCg                                           >  1:1902470/1‑69 (MQ=255)
ttGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCg                                           >  1:1120874/1‑69 (MQ=255)
ttGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCg                                           >  1:1626158/1‑69 (MQ=255)
ttGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCg                                           >  1:452716/1‑69 (MQ=255)
ttGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCg                                           >  1:1012420/1‑69 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:2966460/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:1836567/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:1894079/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:1577275/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:2114626/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:2515439/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:2771271/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:3245005/1‑55 (MQ=255)
              cAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCg                                           >  1:128775/1‑55 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCTCCAGCg                           <  1:436527/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGct                           <  1:1231790/68‑2 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:3047518/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:2340225/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:2486459/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:2452960/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:220979/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:461880/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:66822/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:194393/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:698565/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:862009/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:948191/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:969222/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:1029038/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:1784420/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:1435942/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:145309/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:165848/68‑1 (MQ=255)
                 gCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCg                           <  1:1220726/68‑1 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:2722071/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:2613952/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:2962748/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:1505044/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:3253403/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:2219522/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:21624/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:156272/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:2014941/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:1649374/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:1263114/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:192989/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:1216041/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:835834/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:189028/69‑2 (MQ=255)
                  cTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:613010/69‑2 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:2797545/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:1418566/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:1091366/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:730581/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:2833481/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:1414449/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:511877/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:3385506/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:39521/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:2592400/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:1882873/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:2484855/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:1685374/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:202985/1‑67 (MQ=255)
                   tGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGtac                        >  1:2360816/1‑67 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGGTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:2751788/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCAtt                                   >  1:2879420/1‑53 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCAtt                                   >  1:2174892/1‑53 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1751887/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1094671/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1158354/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1897476/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:3361627/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1306649/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1045247/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:962436/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:1504150/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTc                                  >  1:2826508/1‑54 (MQ=255)
                        gTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGACACACCATCGGTGGCAtt                                   >  1:2720314/1‑53 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:3086977/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:1757676/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:840187/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:83961/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:1943101/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:2891424/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:481757/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:2855702/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:2775710/69‑1 (MQ=255)
                                  tGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATa         <  1:2644916/69‑1 (MQ=255)
                                        tAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAgg   >  1:1808176/1‑69 (MQ=255)
                                        tAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAgg   >  1:3224690/1‑69 (MQ=255)
                                        tAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAgg   >  1:2915202/1‑69 (MQ=255)
                                        tAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAg    >  1:187702/1‑68 (MQ=255)
                                        tAAGCTCACCTGCCAGCGTCACGCCATCGGTGGCATTCACCAGCGta                         <  1:1396773/47‑2 (MQ=37)
                                         aaGCTCACCTGTCAACGTCACACCATCGGTGGCATTCCCCAGCGTCCCATCGCCGTTAAATAGTGAGGc  >  1:795449/1‑69 (MQ=255)
                                         aaGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAGGc  >  1:1560673/1‑69 (MQ=255)
                                         aaGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAGGc  >  1:3356081/1‑69 (MQ=255)
                                         aaGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAGGc  >  1:2263981/1‑69 (MQ=255)
                                                         gTCACACCATCTGTGGCATTCACCAGCGTCCCATCGCCGTTa             <  1:735397/42‑1 (MQ=255)
                                                              |                                               
TTGGAAAGATAAGTCAGGCTGTCAGTTTCAAGGTTGGTATTAAGCTCACCTGTCAACGTCACACCATCGGTGGCATTCACCAGCGTCCCATCGCCGTTAAATAGTGAGGC  >  minE/1469359‑1469468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑