| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,472,914 | T→C | 45.6% | Y166Y (TAT→TAC) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,472,914 | 0 | T | C | 45.6% | 3.7 / 46.3 | 57 | Y166Y (TAT→TAC) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (11/20); new base C (14/12); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGT > minE/1472847‑1472957 | ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:923709/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:2423762/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:2890504/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:1349067/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:1401751/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:1467606/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:3032000/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:322116/68‑1 (MQ=255)ctTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:373434/68‑1 (MQ=255) ctgcCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAgcg > 1:2523633/1‑69 (MQ=255) ttAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGt < 1:1860637/69‑1 (MQ=255) ttAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGt < 1:2169897/69‑1 (MQ=255) ttAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGt < 1:1607636/69‑1 (MQ=255) cagcTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCtt < 1:1345362/69‑1 (MQ=255) aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt < 1:645498/69‑1 (MQ=255) aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt < 1:2288916/69‑1 (MQ=255) aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt < 1:9130/69‑1 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:461088/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:2732554/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:3424308/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:3329346/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:59712/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:88463/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:2865233/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:2723820/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:2698340/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:2619955/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:941483/69‑3 (MQ=255) gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg < 1:2520104/69‑3 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:482390/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:518334/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:3316962/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:3263797/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:1581001/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:830684/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:1291038/69‑1 (MQ=255) aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt < 1:2766269/69‑1 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:1240365/36‑1 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTAt < 1:2494826/36‑1 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTatat > 1:2431270/1‑59 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTTcc > 1:3260865/1‑69 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc > 1:499884/1‑69 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc > 1:1870169/1‑69 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc > 1:2706487/1‑69 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc > 1:52046/1‑69 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGc > 1:3299800/1‑68 (MQ=255) tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATAATCTAGTTGcc > 1:2103267/1‑69 (MQ=255) tatCTGATACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc > 1:436209/1‑69 (MQ=255) tGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCcgcg < 1:2774968/68‑1 (MQ=255) aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCtt > 1:3033767/1‑69 (MQ=255) aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCt < 1:2239792/68‑1 (MQ=255) aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCt < 1:1855917/68‑1 (MQ=255) aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCt < 1:288059/68‑1 (MQ=255) cAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt < 1:1024425/53‑1 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGt > 1:2509229/1‑39 (MQ=37) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:1503019/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:1317792/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:1131132/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:1724071/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:413683/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:194006/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:2042400/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:31396/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:3036428/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:2101454/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:2956252/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:2952269/1‑69 (MQ=255) aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt > 1:2602887/1‑69 (MQ=255) | CTTATGCTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGT > minE/1472847‑1472957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |