Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,472,917 G→A 45.6% E167E (GAG→GAA nrdA → ribonucleoside diphosphate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,472,9170GA45.6% 8.9 / 43.5 57E167E (GAG→GAAnrdAribonucleoside diphosphate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (11/20);  new base A (14/12);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGT  >  minE/1472853‑1472957
                                                                |                                        
ctgcCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAgcg                                      >  1:2523633/1‑69 (MQ=255)
      ttAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGt                                <  1:1607636/69‑1 (MQ=255)
      ttAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGt                                <  1:2169897/69‑1 (MQ=255)
      ttAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGt                                <  1:1860637/69‑1 (MQ=255)
           cagcTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCtt                           <  1:1345362/69‑1 (MQ=255)
                   aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt                   <  1:9130/69‑1 (MQ=255)
                   aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt                   <  1:2288916/69‑1 (MQ=255)
                   aGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt                   <  1:645498/69‑1 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:2520104/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:2732554/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:2619955/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:2698340/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:2723820/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:2865233/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:3329346/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:3424308/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:461088/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:59712/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:88463/69‑3 (MQ=255)
                     gCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCtgg                 <  1:941483/69‑3 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:1291038/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:830684/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:1581001/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:518334/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:482390/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:3316962/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:3263797/69‑1 (MQ=255)
                       aaaTATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGtt               <  1:2766269/69‑1 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTatat                      >  1:2431270/1‑59 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTTcc            >  1:3260865/1‑69 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc            >  1:499884/1‑69 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc            >  1:2706487/1‑69 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc            >  1:52046/1‑69 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc            >  1:1870169/1‑69 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGc             >  1:3299800/1‑68 (MQ=255)
                          tatCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATAATCTAGTTGcc            >  1:2103267/1‑69 (MQ=255)
                          tatCTGATACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGcc            >  1:436209/1‑69 (MQ=255)
                              tGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCcgcg         <  1:2774968/68‑1 (MQ=255)
                                  aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCtt    >  1:3033767/1‑69 (MQ=255)
                                  aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCt     <  1:1855917/68‑1 (MQ=255)
                                  aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCt     <  1:2239792/68‑1 (MQ=255)
                                  aCAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCt     <  1:288059/68‑1 (MQ=255)
                                   cAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCt                   <  1:1024425/53‑1 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGt                                >  1:2509229/1‑39 (MQ=37)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:1503019/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:2602887/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:1317792/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:2101454/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:1131132/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:1724071/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:413683/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:2042400/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:31396/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:3036428/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:2956252/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:2952269/1‑69 (MQ=255)
                                    aGAACCGCGTGACCGGCGAAATCTACGAAAGCGCCCAGTTCCTTTATATTCTGGTTGCCGCGTGCCTGt  >  1:194006/1‑69 (MQ=255)
                                                                |                                        
CTGCCGTTAAGCAGCTGGAAGGCAAATATCTGGTACAGAACCGCGTGACCGGCGAAATCTATGAGAGCGCCCAGTTCCTTTATATTCTAGTTGCCGCGTGCTTGT  >  minE/1472853‑1472957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: