| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 117,272 | G→A | 66.5% | V306V (GTG→GTA) | acnB → | bifunctional aconitate hydratase 2 and 2‑methylisocitrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 117,272 | 0 | G | A | 66.5% | 89.0 / 65.0 | 116 | V306V (GTG→GTA) | acnB | bifunctional aconitate hydratase 2 and 2‑methylisocitrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (16/23); new base A (23/54); total (39/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCG > minE/117219‑117339 | gcgGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCg < 1:2676757/69‑1 (MQ=255)gcgGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCg < 1:2044477/69‑1 (MQ=255) cgGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCg < 1:2783525/68‑1 (MQ=255) gCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGa < 1:1798876/69‑1 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGa > 1:606013/1‑43 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGaa > 1:2456263/1‑69 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGaa > 1:912321/1‑69 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGaa > 1:684576/1‑69 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGaa > 1:391326/1‑69 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGaa > 1:2788260/1‑69 (MQ=255) ccAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGaa > 1:2606422/1‑69 (MQ=255) cAATCGAAGTCGACGTCTCTAACATGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAg > 1:1876357/1‑69 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1013328/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1002129/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1229873/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1049626/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:2345051/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1740886/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:2192513/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:638709/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1062856/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:2478156/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:1495102/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:268761/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGc > 1:2279100/1‑68 (MQ=255) cGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCAGTACAAAGGTGAAGTGc > 1:2338670/1‑68 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGATGTAATTGACGTTTACCCGTACAAAGGTGa < 1:2093928/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCTTACAAAGGTGa < 1:30473/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:2189378/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:2233202/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:1067896/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:1847372/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:3421241/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:1757187/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:513625/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:567188/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:16823/62‑1 (MQ=255) gAAGTCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGa < 1:762244/62‑1 (MQ=255) gTCGACGTCTCTAACATGAACAT‑GGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:1597033/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTATAAAGGTGAAGTGCGTAAcc < 1:1736832/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2613797/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2661592/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:1203199/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2601521/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:1272156/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2784934/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2898698/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2962920/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2992295/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:3046080/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:3066301/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:676563/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:680417/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:743380/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:2591477/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:184174/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:1257059/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:1968140/69‑1 (MQ=255) tCGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc < 1:1415243/69‑1 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:3332488/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:1028191/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:2230965/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:2271534/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:2689380/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:1124003/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:2351554/1‑68 (MQ=255) cGACGTCTCTAACCTGAACATGGGCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAAcc > 1:773299/1‑68 (MQ=255) gTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa > 1:2817642/1‑69 (MQ=255) gTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa < 1:713504/69‑1 (MQ=255) gTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa < 1:706617/69‑1 (MQ=255) gTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa > 1:1016208/1‑69 (MQ=255) gTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa > 1:329178/1‑69 (MQ=255) gTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGaa > 1:1861084/1‑69 (MQ=255) ggCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCg > 1:2298003/1‑69 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:2520919/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:758643/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:12840/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:423045/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:1576989/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:1550953/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:145506/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:3292953/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:3340634/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:3257488/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:2692630/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:2291252/69‑2 (MQ=255) ggCGACGTAATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGca < 1:2969479/69‑2 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2520157/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2397618/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2755614/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:220634/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2089277/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2044244/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1873373/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1633749/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:3186796/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1699686/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1746714/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:413944/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:3269382/69‑1 (MQ=255) | GCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCG > minE/117219‑117339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |