| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,473,718 | T→C | 69.7% | R434R (CGT→CGC) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,473,718 | 0 | T | C | 69.7% | 89.9 / 41.8 | 92 | R434R (CGT→CGC) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (23/5); new base C (27/37); total (50/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TTCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACG > minE/1473657‑1473785 | ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:1438146/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:1959736/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:1642087/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:1429088/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:876346/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:1184912/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:93876/69‑1 (MQ=255)ttCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:2449418/69‑1 (MQ=255) tCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:2685359/68‑1 (MQ=255) tCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:2135828/68‑1 (MQ=255) cAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTa < 1:2933679/67‑1 (MQ=255) gCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAg < 1:2762100/69‑1 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCt > 1:1238697/1‑52 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2833635/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1873567/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:3077950/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2973983/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:41393/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1606730/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:461996/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:973555/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1418449/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2323751/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2338850/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2844473/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2875972/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2038712/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:410445/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:2452120/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:3194555/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:3307285/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1677497/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1486226/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:791723/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1087807/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGACCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:1012934/1‑69 (MQ=255) aTACCCATAGCCCGTTTGATCCGAACATCGCGCCAGTGCGCCAGTCTAACCTGTGCCTGGAAATCGccc > 1:271458/1‑69 (MQ=255) cccATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAg > 1:2236545/1‑63 (MQ=255) cccATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAg > 1:1154765/1‑63 (MQ=255) cccATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTg > 1:660970/1‑68 (MQ=255) cccATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTg > 1:2523756/1‑68 (MQ=255) cccATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTAAGTCTAACCTGTGCCTGGAGATAg > 1:754008/1‑63 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGgaga > 1:1808300/1‑54 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:1072198/1‑69 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:42970/1‑69 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:1142061/1‑69 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:1836035/1‑69 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:1965440/1‑69 (MQ=255) gCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCa > 1:2329619/1‑69 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2560429/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:64844/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:553515/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:1585653/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:717353/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:386730/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:128448/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:3383744/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:175372/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:3302096/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:323422/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:182347/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:1257023/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:1990679/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2235213/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:879332/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2622692/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2624518/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2644476/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2671960/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:231842/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2692080/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:812917/43‑1 (MQ=255) ttGATCCGGCCATCGCGCCAGTGCGCCAGTCTAACCTGTGCCt < 1:2076837/43‑1 (MQ=255) tGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCg > 1:1383738/1‑67 (MQ=255) tGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCg > 1:2047072/1‑67 (MQ=255) tGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGc > 1:772962/1‑68 (MQ=255) tGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGc > 1:776985/1‑68 (MQ=255) gcgcCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAac < 1:831127/69‑1 (MQ=255) gcgcCAGTGCGTCAGTCTAACCTGTGCCTGAAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAac > 1:3201581/1‑69 (MQ=255) gcCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTc < 1:122807/64‑1 (MQ=255) gcCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTc < 1:3172684/64‑1 (MQ=255) ccAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTc < 1:362761/63‑1 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:839176/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:744055/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:2520225/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:1458026/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:1636871/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:357538/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:3270310/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:3187965/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:2061523/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:2175193/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:253516/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGaa > 1:2475901/1‑69 (MQ=255) gcgcCAGTCTAACCTGTGCCTGGAAATCCCCCTGCCGACCAAACCGCTGAACGACGTCAacga > 1:1744900/1‑63 (MQ=255) cGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACGGCTGAACGACGTCAACGACGAGaa > 1:843672/1‑68 (MQ=255) cGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAAc > 1:2962988/1‑69 (MQ=255) cGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAAc > 1:2905622/1‑69 (MQ=255) cGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAAc > 1:2785085/1‑69 (MQ=255) cGTCAGTCTAAC‑‑GTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACg > 1:1126750/1‑68 (MQ=255) | TTCAGAACGTTGACCACTGCAATACCCATAGCCCGTTTGATCCGGCCATCGCGCCAGTGCGTCAGTCTAACCTGTGCCTGGAGATAGCCCTGCCGACCAAACCGCTGAACGACGTCAACGACGAGAACG > minE/1473657‑1473785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |