| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,474,576 | C→T | 34.3% | T720T (ACC→ACT) | nrdA → | ribonucleoside diphosphate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,474,576 | 0 | C | T | 34.3% | 70.0 / 48.8 | 90 | T720T (ACC→ACT) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (19/40); new base T (16/15); total (35/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-02 | |||||||||||
ACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGC > minE/1474509‑1474638 | aCTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:88424/69‑1 (MQ=255)aCTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:1390977/69‑1 (MQ=255)aCTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:1163326/69‑1 (MQ=255)aCTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:1791737/69‑1 (MQ=255)aCTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:1244570/69‑1 (MQ=255)aCTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:1445629/69‑1 (MQ=255) cTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:1321509/68‑1 (MQ=255) aCGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGcc > 1:2604203/1‑68 (MQ=255) ccGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg < 1:3421585/61‑1 (MQ=255) ccGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACaaa < 1:355689/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:2521474/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:2581494/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:151977/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:1310183/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:250757/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:1725847/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:1753154/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:2614312/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:551899/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:2722382/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:1194577/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:2867767/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:3227849/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCg < 1:2196227/69‑1 (MQ=255) cACGTTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCAACAAATTCg < 1:3346393/69‑1 (MQ=255) aCGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCgg > 1:2114635/1‑69 (MQ=255) aaaaGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGta < 1:899070/69‑1 (MQ=255) aaaGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGtat > 1:901339/1‑69 (MQ=255) aaaGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGtat > 1:1814208/1‑69 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAg < 1:3045434/69‑1 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAg < 1:3315327/69‑1 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAg < 1:2217467/69‑1 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAg < 1:2051991/69‑1 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAg < 1:914971/69‑1 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTACCAg < 1:2764725/69‑1 (MQ=255) ccGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGGCAAAACACTGTATTATCAg < 1:2388969/69‑1 (MQ=255) cGATGCAGCAGTTGCTGAAAGCCCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:2714516/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACaa > 1:1334953/1‑40 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:2941854/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:575489/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:1419704/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:2510798/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:1642182/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:2478048/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:2391938/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:1168617/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:2301066/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:3065145/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:1138146/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:97155/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:1008425/1‑52 (MQ=255) cGATGCAGCAGTTGCTGAAAGACCTGCTCACTGCCTACAAATTCGGGGTCaa > 1:86035/1‑52 (MQ=255) gcagcaGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAacac > 1:2362489/1‑69 (MQ=255) gcagcaGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAacac > 1:2645844/1‑69 (MQ=255) cagcagTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACAcc < 1:1249096/69‑1 (MQ=255) cagTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGt < 1:1401865/69‑1 (MQ=255) cagTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGt < 1:1004866/69‑1 (MQ=255) cagTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGt < 1:511700/69‑1 (MQ=255) cagTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGt < 1:2083213/69‑1 (MQ=255) cagTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGt < 1:2426712/69‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGt < 1:2590316/47‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGt < 1:874723/47‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGt < 1:2296319/47‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGt < 1:2357268/47‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGt < 1:2469571/47‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGt < 1:1427862/47‑1 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGAc > 1:1588760/1‑67 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACgg > 1:1185558/1‑69 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACgg > 1:2783432/1‑69 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACgg > 1:3174526/1‑69 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACgg > 1:3188399/1‑69 (MQ=255) gCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACgg > 1:648662/1‑69 (MQ=255) tGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:342786/68‑1 (MQ=255) tGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:1005816/68‑1 (MQ=255) tGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:3037925/68‑1 (MQ=255) tGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:2833686/68‑1 (MQ=255) tGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:2674773/68‑1 (MQ=255) tGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:1786178/68‑1 (MQ=255) tGAAACACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgc < 1:3344636/68‑1 (MQ=255) aaGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgcgc > 1:3242438/1‑67 (MQ=255) aaGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgcgc > 1:2684420/1‑67 (MQ=255) aaGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgcgc > 1:1961720/1‑67 (MQ=255) aaGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgcgc > 1:1779499/1‑67 (MQ=255) aaGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgcgc > 1:1292786/1‑67 (MQ=255) aaGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGgcg > 1:1128894/1‑66 (MQ=255) ccTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTGAAGa < 1:2624820/69‑1 (MQ=255) ccTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTGAAGa < 1:1908977/69‑1 (MQ=255) ccTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTGAAGa < 1:1896817/69‑1 (MQ=255) ccTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTGAAGa < 1:985943/69‑1 (MQ=255) cTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTTAAGACGc > 1:2190109/1‑68 (MQ=255) | ACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCAAAACACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGC > minE/1474509‑1474638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |