| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,298 | T→C | 31.4% | R121R (CGT→CGC) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,298 | 0 | T | C | 31.4% | 57.6 / 34.3 | 70 | R121R (CGT→CGC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base T (24/24); new base C (16/6); total (40/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
AACTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGAT > minE/1475246‑1475355 | aaCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:532536/69‑1 (MQ=255)aaCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:932458/69‑1 (MQ=255)aaCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:1506967/69‑1 (MQ=255) aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:1573754/68‑1 (MQ=255) aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCAt > 1:566058/1‑69 (MQ=255) aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCAt > 1:3033706/1‑69 (MQ=255) aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCAt > 1:819645/1‑69 (MQ=255) gAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCa > 1:3020931/1‑59 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATc > 1:3016524/1‑61 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCg > 1:1117877/1‑66 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:292721/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3334880/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:2235059/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3301467/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:807455/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:156439/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3011036/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1499880/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:2903236/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1366073/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1349841/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1229218/1‑69 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTa > 1:2617964/1‑68 (MQ=255) aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTa > 1:1461213/1‑68 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGt > 1:1717461/1‑43 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:2722969/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:3314193/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:759064/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:2086212/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1998017/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:765666/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1412063/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1033508/1‑69 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:611342/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:730469/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:2819949/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:852922/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:856927/66‑1 (MQ=255) agAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:310449/66‑1 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTaa > 1:1698974/1‑45 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:1824576/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:2279825/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:2280113/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:431868/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:680733/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3381076/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:334982/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:252429/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3241546/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3085005/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:1638149/1‑69 (MQ=255) aCGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGAc < 1:22874/69‑1 (MQ=255) aCGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGAc < 1:1019163/69‑1 (MQ=255) ttcattcCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGat < 1:254314/68‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:967296/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:734348/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2257508/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:894677/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:450983/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:419739/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:3410452/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2287513/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2699/40‑1 (MQ=255) attcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:154825/38‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:195169/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1864840/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:3136446/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:3042000/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1574208/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:2689655/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1270911/39‑1 (MQ=255) | AACTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGAT > minE/1475246‑1475355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |