| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,316 | T→C | 21.5% | I127I (ATT→ATC) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,316 | 0 | T | C | 21.5% | 132.8 / 27.9 | 97 | I127I (ATT→ATC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base T (50/26); new base C (15/6); total (65/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCAGAA > minE/1475253‑1475381 | aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCg > 1:1117877/1‑66 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:807455/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:156439/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3334880/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:2235059/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1499880/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3301467/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1366073/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1349841/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3011036/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:292721/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1229218/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:2903236/1‑69 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTa > 1:2617964/1‑68 (MQ=255)aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTa > 1:1461213/1‑68 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1033508/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:3314193/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:2086212/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1998017/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:2722969/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1412063/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:765666/1‑69 (MQ=255) cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:759064/1‑69 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:856927/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:852922/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:730469/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:2819949/66‑1 (MQ=255) tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:611342/66‑1 (MQ=255) agAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:310449/66‑1 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTaa > 1:1698974/1‑45 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:1824576/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3381076/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:680733/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:334982/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3085005/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:431868/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:1638149/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:2279825/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:2280113/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3241546/1‑69 (MQ=255) ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:252429/1‑69 (MQ=255) aCGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGAc < 1:1019163/69‑1 (MQ=255) aCGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGAc < 1:22874/69‑1 (MQ=255) ttcattcCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGat < 1:254314/68‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:894677/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:967296/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:734348/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:3410452/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:419739/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:450983/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2699/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2257508/40‑1 (MQ=255) tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2287513/40‑1 (MQ=255) attcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:154825/38‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:2689655/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1864840/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:195169/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1574208/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:3136446/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:3042000/39‑1 (MQ=255) cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1270911/39‑1 (MQ=255) atCATTGGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:436973/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGa > 1:1514611/1‑44 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGAt > 1:2721715/1‑65 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:849659/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:1516600/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:853666/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:1207176/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:1548866/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:556442/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:524844/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:1331768/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:1684038/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:3431334/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:2914461/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:2137752/1‑68 (MQ=255) atCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCa > 1:2303069/1‑68 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATc < 1:166735/47‑1 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATc < 1:1594168/47‑1 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATc < 1:401839/47‑1 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATc < 1:2677872/47‑1 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATc < 1:2639803/47‑1 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATc < 1:1505897/47‑1 (MQ=255) tCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCAGa > 1:1339291/1‑69 (MQ=255) cATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCAGaa > 1:2696951/1‑69 (MQ=255) cATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCAGaa > 1:845629/1‑69 (MQ=255) cATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCAGaa > 1:624757/1‑69 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:3140183/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:3243190/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:1385711/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:2265984/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:682338/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:850287/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:3328682/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:610992/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:211012/1‑60 (MQ=255) aTTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAg > 1:3379305/1‑60 (MQ=255) | AACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGATATCGTCACCAACGAGCAGATCCAGAA > minE/1475253‑1475381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |