| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,709 | C→T | 36.3% | D258D (GAC→GAT) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,709 | 0 | C | T | 36.3% | 34.2 / 30.2 | 55 | D258D (GAC→GAT) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base C (11/24); new base T (13/7); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTT > minE/1475647‑1475769 | gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt < 1:1038486/69‑1 (MQ=255)gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt < 1:1251946/69‑1 (MQ=255) cgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga < 1:118851/69‑1 (MQ=255) cgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga < 1:490062/69‑1 (MQ=255) gCACCTGCCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:1545754/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:3376370/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:2045880/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:2613154/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:2877702/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:2934152/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:1226463/69‑1 (MQ=255) gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:1003043/69‑1 (MQ=255) cATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTa > 1:1451685/1‑67 (MQ=255) tGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAgg > 1:1288012/1‑69 (MQ=255) tGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAgg > 1:3136153/1‑69 (MQ=255) tGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAgg > 1:2207443/1‑69 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGTATGATCCGGAGATGGCGGAAATTGcc > 1:291931/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:3053007/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:3173641/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:386241/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:2218700/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1028374/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:971035/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1214541/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1230536/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1270838/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1382930/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1765327/1‑52 (MQ=255) gCTGAATCTGCTGCGCAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGcc > 1:1672748/1‑52 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:1939024/58‑1 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg > 1:3307807/1‑58 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg > 1:301190/1‑58 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:819833/58‑1 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg > 1:1439314/1‑58 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg > 1:1545109/1‑58 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg > 1:2432094/1‑58 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:541220/58‑1 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:769841/58‑1 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:1893055/58‑1 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:1976293/58‑1 (MQ=255) tCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCt > 1:1802165/1‑68 (MQ=255) ctgctgCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGc > 1:2361399/1‑58 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGCCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:795756/68‑1 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGCCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:990304/68‑1 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGCCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:3258232/68‑1 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGCCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:1426151/68‑1 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGCCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:1192164/68‑1 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTGCCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:1034958/68‑1 (MQ=255) cAGCGGCGCGGATGATCCGGAGATGGCGGAAATTACCGAAGAATGTAAGCAGGAGTGCTATGACCtgt < 1:563799/68‑1 (MQ=255) ggcgCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTaat < 1:3429027/45‑2 (MQ=255) ggcgCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:3032749/45‑1 (MQ=255) ggcgCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:2083532/45‑1 (MQ=255) ggcgCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAg < 1:1938563/45‑1 (MQ=255) ggcgCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCtgtttgtt < 1:2155817/69‑1 (MQ=255) ggcgCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCtgtttgtt < 1:95354/69‑1 (MQ=255) | GACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTT > minE/1475647‑1475769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |