| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,817 | C→T | 19.5% | D294D (GAC→GAT) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,817 | 0 | C | T | 19.5% | 163.1 / 26.1 | 107 | D294D (GAC→GAT) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base C (26/60); new base T (7/14); total (33/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
CTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTA > minE/1475754‑1475884 | cTATGACGTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTc > 1:142934/1‑69 (MQ=255)cTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTc > 1:2381828/1‑69 (MQ=255)cTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTc > 1:2357149/1‑69 (MQ=255) aTGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCg > 1:574443/1‑68 (MQ=255) aTGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCg > 1:2971407/1‑68 (MQ=255) aTGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCg > 1:1600461/1‑68 (MQ=255) tgttCAGTCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACgg < 1:2553268/54‑1 (MQ=255) tgttCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACgg < 1:2505855/54‑1 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTg > 1:2617094/2‑67 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTg > 1:1469198/2‑67 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTGa > 1:87514/2‑68 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTGa > 1:2693165/2‑68 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTGa > 1:2272398/2‑68 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTGa > 1:1379426/2‑68 (MQ=255) ccAGGCGGCACAGCAAGAGAAAGACTGGGCGGATTATCTGTTCCGTGATGGTTCGATGATTGGTCTGa > 1:1126693/2‑68 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGTATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATa < 1:1511124/68‑1 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGTTCTGAATa < 1:1859432/68‑1 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATa < 1:82896/68‑1 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATa < 1:783232/68‑1 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATa < 1:2617681/68‑1 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATa < 1:567001/68‑1 (MQ=255) ggCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATa < 1:1843541/68‑1 (MQ=255) gCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATaaa < 1:1028597/69‑1 (MQ=255) gCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATaaa < 1:1320838/69‑1 (MQ=255) gCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATaaa < 1:3136420/69‑1 (MQ=255) gCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATaaa < 1:755952/69‑1 (MQ=255) gCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTATGAATa < 1:3372966/67‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:1208362/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:389885/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:1185060/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:632681/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:2632634/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:2810064/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:785087/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGa < 1:2739810/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGACTGGTCTGAATAAAGa < 1:1079293/69‑1 (MQ=255) aGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCCACGGTTCGATGATTGGTCTGAATAAAGa < 1:3049423/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATTAAGACa < 1:3793/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2694006/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2509102/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2788111/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:3238037/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:3399947/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:356543/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:49979/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:681948/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2475949/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:137718/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2361539/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2006339/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2338374/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2311848/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:1338844/69‑1 (MQ=255) cTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:1611132/69‑1 (MQ=255) tCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACa < 1:2297892/68‑1 (MQ=255) cAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACAtt < 1:1904444/69‑1 (MQ=255) agaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCgatga < 1:2807167/42‑1 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:3042603/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2066153/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2017231/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2860241/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:3426843/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2332742/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:1530382/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:1461382/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:1303355/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2409953/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2492153/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCCGAATAAAGACATTCTCTGcc > 1:1186594/1‑68 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:1536313/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:3222564/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:2781323/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:2670709/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:2174150/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:3428180/68‑1 (MQ=255) tgATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:343183/68‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:2633919/69‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:3187083/69‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGGCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:2234381/69‑1 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:2886230/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:1049193/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:3133081/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:84037/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTATCTGCCAGTACGTTGAATACAt > 1:1340160/1‑69 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:179388/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1474872/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:74737/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:740642/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:807894/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1575544/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:2744610/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1793799/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1842255/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:3270247/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:3254579/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:2988126/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:2043673/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:3004880/69‑1 (MQ=255) aCGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTa < 1:112820/69‑1 (MQ=255) aCGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTa < 1:30951/69‑1 (MQ=255) | CTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTA > minE/1475754‑1475884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |