| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 117,332 | G→A | 51.8% | A326A (GCG→GCA) | acnB → | bifunctional aconitate hydratase 2 and 2‑methylisocitrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 117,332 | 0 | G | A | 51.8% | ‑3.4 / 47.1 | 54 | A326A (GCG→GCA) | acnB | bifunctional aconitate hydratase 2 and 2‑methylisocitrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (5/21); new base A (18/10); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
GGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCG > minE/117264‑117392 | ggCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCg > 1:2298003/1‑69 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:10862/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2520157/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2755614/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2397618/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:220634/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2089277/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:2044244/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1873373/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:3186796/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1746714/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:3269382/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1633749/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:1699686/69‑1 (MQ=255) tGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCg < 1:413944/69‑1 (MQ=255) gACGTTTACCCGTATAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:2978915/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGa > 1:2942413/1‑65 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:2594312/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:973022/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:1041813/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:824088/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:655810/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:618364/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:2876998/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:2898345/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:488164/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:3158091/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:3345935/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:2488664/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:1174130/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:1983068/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:1826355/1‑68 (MQ=255) gACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCAACCTTCGAACt > 1:1501352/1‑68 (MQ=255) tACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAAcc > 1:1271015/1‑69 (MQ=255) gTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCtgattg > 1:306745/1‑69 (MQ=255) aCCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAgtgcgtg > 1:3026210/1‑69 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:2522477/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:112077/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:928725/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:1251996/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:632081/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:1263511/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:334026/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:2183865/44‑1 (MQ=255) cGAAACCGGCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:2673070/44‑1 (MQ=255) cGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTTCTGGTGGCCg < 1:499849/69‑1 (MQ=255) cGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCg < 1:402081/69‑1 (MQ=255) cGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCg < 1:528379/69‑1 (MQ=255) cGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCg < 1:2100391/69‑1 (MQ=255) cGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCg < 1:2784781/69‑1 (MQ=255) cGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCg < 1:2534991/69‑1 (MQ=255) gCGAACTGCTGGCAACCTTCGAACTGAAAACCGACg < 1:1465589/36‑1 (MQ=255) ctgctgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCg > 1:1663861/1‑69 (MQ=255) ctgctgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCg > 1:1936645/1‑69 (MQ=255) tgGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAg < 1:2075456/42‑1 (MQ=255) | GGCGACGTGATTGACGTTTACCCGTACAAAGGTGAAGTGCGTAACCACGAAACCGGCGAACTGCTGGCGACCTTCGAACTGAAAACCGACGTGCTGATTGATGAAGTGCGTGCTGGTGGCCGTATTCCG > minE/117264‑117392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |