| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,475,847 | T→C | 42.7% | I304I (ATT→ATC) | nrdB → | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,475,847 | 0 | T | C | 42.7% | 24.4 / 68.3 | 89 | I304I (ATT→ATC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base T (37/14); new base C (23/15); total (60/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
CAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCA > minE/1475779‑1475915 | cAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACAtt < 1:1904444/69‑1 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2492153/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:957682/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:3426843/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:3042603/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2860241/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2409953/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2332742/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2066153/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:2017231/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:1530382/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:1461382/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGcc > 1:1303355/1‑68 (MQ=255) gaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCCGAATAAAGACATTCTCTGcc > 1:1186594/1‑68 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:2174150/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:3428180/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:3222564/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:1536313/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:2781323/68‑1 (MQ=255) cGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGaa < 1:2670709/68‑1 (MQ=255) tgATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:343183/68‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:3187083/69‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:3135101/69‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:2633919/69‑1 (MQ=255) ggATTATCTGTTCCGCGACGGTTCGATGATTGGGCTGAATAAAGACATTCTCTGCCAGTACGTTGAATa < 1:2234381/69‑1 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACg > 1:273982/1‑59 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:3133081/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:1049193/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:2886230/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:1456852/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACAt > 1:84037/1‑69 (MQ=255) ttATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTATCTGCCAGTACGTTGAATACAt > 1:1340160/1‑69 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:179388/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:2988126/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:3004880/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:2744610/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:740642/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:74737/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1575544/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1793799/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:807894/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1474872/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:1842255/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:2043673/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:3270247/69‑1 (MQ=255) ccGTGATGGTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:3254579/69‑1 (MQ=255) aCGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTa < 1:30951/69‑1 (MQ=255) aCGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTa < 1:112820/69‑1 (MQ=255) tggTTCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatat < 1:947035/62‑1 (MQ=255) tCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:78234/1‑68 (MQ=255) tCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:1629239/1‑68 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAatatg > 1:2141156/1‑59 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:724350/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:3101011/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:498485/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1431136/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:3092019/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:608056/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:516775/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:343112/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1192522/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1933824/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1275600/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1259239/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:2143846/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1285624/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:826704/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:2332090/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:660279/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:2551775/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1781240/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:1735170/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:117580/1‑62 (MQ=255) tCGATGATTGGTCTGAATAAAGACATCCTCTGCCAGTACGTTGAATACATCACCAATATCCg > 1:2910162/1‑62 (MQ=255) cGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgcagg < 1:178452/69‑1 (MQ=255) atgatTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgcaggca > 1:316579/1‑69 (MQ=255) cTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAat > 1:1112614/1‑45 (MQ=255) cTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAat > 1:3335679/1‑45 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:1997924/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:876803/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:227317/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:2367571/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:2731578/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:173984/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:674607/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:2951261/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:643735/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:1148506/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:54455/1‑49 (MQ=255) aaGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATgca > 1:1504305/1‑49 (MQ=255) tctcTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCa < 1:2710666/69‑1 (MQ=255) tctcTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCa < 1:2422212/69‑1 (MQ=255) tctcTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCa < 1:132633/69‑1 (MQ=255) tctcTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCa < 1:1473337/69‑1 (MQ=255) tctcTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCa < 1:2210970/69‑1 (MQ=255) tctcTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCa < 1:1322298/69‑1 (MQ=255) | CAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCA > minE/1475779‑1475915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |