| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,476,848 | A→G | 29.8% | Y59Y (TAT→TAC) | inaA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,476,848 | 0 | A | G | 29.8% | 157.9 / 80.4 | 164 | Y59Y (TAT→TAC) | inaA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (38/77); new base G (25/24); total (63/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 164 total aligned reads displayed.
GACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTT > minE/1476782‑1476899 | gACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGAt > 1:3127325/1‑68 (MQ=255)gACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATa > 1:3162076/1‑69 (MQ=255) acCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACg < 1:3099026/69‑1 (MQ=255) acCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACg < 1:1288778/69‑1 (MQ=255) cccGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACgg < 1:383781/69‑1 (MQ=255) ccGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGa < 1:2454208/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:2263306/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:2615875/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:722479/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:1449878/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:2414003/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:1888500/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:395383/69‑1 (MQ=255) ccggTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1465193/4‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATg > 1:2675778/1‑67 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2099692/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:3361548/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:3318917/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:3326917/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:551902/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2418077/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:569429/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2671850/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1144766/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1079498/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1014871/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGCCCGAACGGATAACGTACGGa > 1:1956183/1‑64 (MQ=255) ccAGTTCTTTAATTACAGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAAc > 1:1213894/1‑57 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2874918/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2534793/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2548282/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:3270018/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2395734/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2383904/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:3272496/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2275822/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:211396/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:849239/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:632142/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:1595978/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:609291/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:419298/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:1922779/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:1924772/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:3382582/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:3354258/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:333238/63‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:237834/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:1201741/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:2631103/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:3058064/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:815436/69‑1 (MQ=255) tttAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:1424201/68‑1 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTAc > 1:2201023/1‑50 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:890617/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2923060/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2925852/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:674879/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:3119936/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:488046/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:360816/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:3429892/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2014976/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1067713/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2753627/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1181977/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2558846/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1897697/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1844466/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2408989/1‑62 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:515495/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2025590/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2052899/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:1869818/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:1643023/69‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:1016622/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:1919081/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:534412/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:855674/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:3231371/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2233755/68‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:2138206/69‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:2821991/69‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:1091680/69‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:1612426/69‑1 (MQ=255) aCCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATgcgc > 1:1118002/1‑69 (MQ=255) cGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATATAGCtt < 1:1749626/69‑1 (MQ=255) cGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCtt < 1:2470552/69‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2309578/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:1724869/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:1652050/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:2844090/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:3094033/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:1507196/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:493357/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:394331/1‑69 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2711979/46‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2800662/46‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:1999717/46‑1 (MQ=255) ggATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:264433/45‑1 (MQ=255) | GACACCGGCCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTT > minE/1476782‑1476899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |