| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,476,857 | G→A | 29.1% | S56S (TCC→TCT) | inaA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,476,857 | 0 | G | A | 29.1% | 174.9 / 74.3 | 164 | S56S (TCC→TCT) | inaA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (48/68); new base A (24/24); total (72/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01 | |||||||||||
Only 100 of 166 total aligned reads displayed.
CCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCA > minE/1476790‑1476924 | cccGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACgg < 1:383781/69‑1 (MQ=255) ccGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGa < 1:2454208/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:1893331/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:722479/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:2615875/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:3071948/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:422885/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:2701116/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:2826800/69‑1 (MQ=255) tCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa < 1:1449878/69‑1 (MQ=255) ccggTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1465193/4‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:3361548/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:3318917/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2099692/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2671850/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2393019/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1014871/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2418077/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:2545247/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:551902/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:88029/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGa > 1:1144766/1‑68 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTAAGGAAtta > 1:33980/1‑66 (MQ=255) ccAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGCCCGAACGGATAACGTACGGa > 1:1956183/1‑64 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:632142/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2275822/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:609291/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2874918/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:211396/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:1595978/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:2395734/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:407740/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:3272496/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:333238/63‑1 (MQ=255) gTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAAt < 1:3382582/63‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:91439/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:815436/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:237834/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:2508617/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:1201741/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:3058064/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAgat < 1:1844751/69‑1 (MQ=255) cTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAg > 1:2002178/1‑67 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2753627/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:488046/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2899739/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2925852/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2558846/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:360816/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2415880/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1923958/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1288660/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1181977/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2348270/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:1067713/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGGTAACGTACAGAATGAAACAg > 1:2408989/1‑62 (MQ=255) aTTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGatgatg < 1:1348798/68‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2800688/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:1643023/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2052899/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:1869818/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2025590/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:1959069/69‑1 (MQ=255) tACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:3126239/69‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:855674/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:534412/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:497831/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:1919081/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGaa < 1:1016622/55‑1 (MQ=255) aCCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGt < 1:2233755/68‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:1903601/69‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:2138206/69‑1 (MQ=255) gCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATg < 1:1612426/69‑1 (MQ=255) aCCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATgcgc > 1:1118002/1‑69 (MQ=255) cGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCtt > 1:3116099/1‑69 (MQ=255) cGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCtt < 1:1840143/69‑1 (MQ=255) cGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCtt > 1:635297/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:3233330/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2642753/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:3353384/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2309578/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:1724869/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:1652050/60‑1 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:566520/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:394331/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCttt > 1:1717513/1‑69 (MQ=255) gTTGGTCGGCCGAACGGATAACGTACGGAACGAAACAGATGATGCGTCATGCGCTTtaca < 1:1084408/60‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:3219413/46‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2711979/46‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2259193/46‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2800662/46‑1 (MQ=255) cGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:2034597/46‑1 (MQ=255) ggATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTtaca < 1:264433/45‑1 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:519285/1‑69 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:517725/1‑69 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:793433/1‑69 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:822083/1‑69 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:3284216/1‑69 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:1061441/1‑69 (MQ=255) tACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTg > 1:2523243/1‑69 (MQ=255) | CCCGTTCCAGTTCTTTAATTACCGCAACCTCACGGACAATCGTTGGTCGGCCGAACGGATAACGTACGGAATGAAACAGATGATGCGTCATGCGCTTTACATACAGCTTTTTGCCGTTGCGCTCGACGCATTGCA > minE/1476790‑1476924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |