| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,478,321 | G→A | 47.4% | Y81Y (TAC→TAT) | glpQ ← | periplasmic glycerophosphodiester phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,478,321 | 0 | G | A | 47.4% | 13.4 / 103.0 | 118 | Y81Y (TAC→TAT) | glpQ | periplasmic glycerophosphodiester phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (46/16); new base A (35/21); total (81/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
GTAGTAACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAT > minE/1478255‑1478382 | gtagtaACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGt < 1:485365/68‑1 (MQ=255)gtagtaACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTa < 1:2563930/69‑1 (MQ=255)gtagtaACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTa < 1:2933992/69‑1 (MQ=255)gtagtaACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTa < 1:2206673/69‑1 (MQ=255) tagtaACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTa < 1:1935810/68‑1 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:2226249/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:2892917/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:537388/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:181016/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:3193399/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:1512131/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCTCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTc > 1:3013943/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:389865/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:2567180/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:2036325/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:2075280/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:3266220/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:2084707/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:682233/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:147570/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:767037/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:3027977/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:2825488/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:244457/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:2527226/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:250381/1‑67 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATg > 1:1679023/1‑66 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATg > 1:901444/1‑66 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGt < 1:72400/68‑1 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTc > 1:2153165/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTc > 1:1932754/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTc > 1:1972852/1‑69 (MQ=255) aCGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTc > 1:798359/1‑69 (MQ=255) aCGACAGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATgg > 1:905639/1‑67 (MQ=255) cGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGc > 1:3268596/1‑68 (MQ=255) cGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGc > 1:1817310/1‑68 (MQ=255) cGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGc > 1:2785742/1‑68 (MQ=255) cgcgcCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGaacaac < 1:431547/68‑1 (MQ=255) cgcgcCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGaacaac < 1:792119/68‑1 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:3430518/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:2580908/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:2681611/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:2745468/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:2487432/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:3066284/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:2186867/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:1761670/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:1735514/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:820304/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:128662/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:1105758/1‑69 (MQ=255) ccGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTgtcgt > 1:1056507/1‑69 (MQ=255) tgAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:959685/68‑1 (MQ=255) ggAAACGATTGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2750607/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2970539/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:37479/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:1998217/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:1709032/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:548923/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:1138316/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:1411602/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:785413/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2600779/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:132948/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:1317201/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2607046/69‑1 (MQ=255) ggAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2723099/69‑1 (MQ=255) gAAACGATCGGCAACATCAGTAACACGATGGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:625990/68‑1 (MQ=255) gAAACGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2668744/68‑1 (MQ=255) aaaGGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:1918503/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtttt > 1:2463386/1‑68 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCttt > 1:852224/1‑68 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCttt > 1:1644553/1‑68 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCttt > 1:3021050/1‑68 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCttt > 1:2298165/1‑68 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCttt > 1:2246555/1‑68 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt > 1:3100614/1‑67 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:3432326/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2856186/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2887505/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:1368777/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2918570/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2534616/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:683833/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:1669331/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:62362/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2212891/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:488718/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:1067789/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:1968120/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2130923/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:2080745/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:371344/1‑69 (MQ=255) aaaCGATCGGCAACATCAGTAACACGATCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCTTTg > 1:926776/1‑69 (MQ=255) gATCTAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAt < 1:3254477/69‑1 (MQ=255) gATCGAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAt < 1:1013334/69‑1 (MQ=255) gATCGAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAt < 1:699228/69‑1 (MQ=255) gATCGAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAt < 1:4487/69‑1 (MQ=255) gATCGAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAt < 1:272239/69‑1 (MQ=255) aTCGAGATAATGGTCATGCAGAACAACCAGATGGTCGTCtt < 1:2000381/41‑1 (MQ=38) | GTAGTAACGACCGTCTTTGCGCGCCCGATCCGGGAAACGATCGGCAACATCAGTAACACGATCGAGGTAATGGTCATGCAGAACAACCAGATTGTCGTCTTTGGTCATCACCAAATCCTGTTCCAGAT > minE/1478255‑1478382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |