| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,478,949 | A→G | 42.8% | V326V (GTT→GTC) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,478,949 | 0 | A | G | 42.8% | 3.5 / 18.0 | 28 | V326V (GTT→GTC) | glpT | sn‑glycerol‑3‑phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base A (13/3); new base G (8/4); total (21/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGT > minE/1478900‑1479011 | ggTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGt < 1:1190026/69‑1 (MQ=255)ggTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGt < 1:1458410/69‑1 (MQ=255)ggTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGt < 1:672290/69‑1 (MQ=255) aTCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGt < 1:1515840/64‑1 (MQ=255) aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:1794214/1‑69 (MQ=255) aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:3083280/1‑69 (MQ=255) aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:2695186/1‑69 (MQ=255) aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:735796/1‑69 (MQ=255) aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:945449/1‑69 (MQ=255) ggATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACAACGAAAGACtt > 1:246290/2‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGccc > 1:1522590/1‑48 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGaaa > 1:1734312/1‑64 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:1674711/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:692577/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:563778/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:3101852/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:3197476/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:2664932/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:596511/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:3224017/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:1043853/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:2505229/1‑69 (MQ=255) aTAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga > 1:259817/1‑69 (MQ=255) aTAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga > 1:2025565/1‑69 (MQ=255) aTAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga > 1:1386960/1‑69 (MQ=255) aaaGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt < 1:978088/69‑1 (MQ=255) aaGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt < 1:747628/68‑1 (MQ=255) aaGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt < 1:1313634/68‑1 (MQ=255) | GGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGT > minE/1478900‑1479011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |