| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,478,976 | G→A | 41.1% | F317F (TTC→TTT) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,478,976 | 0 | G | A | 41.1% | 18.3 / 33.5 | 51 | F317F (TTC→TTT) | glpT | sn‑glycerol‑3‑phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base G (19/11); new base A (6/15); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCA > minE/1478913‑1479033 | aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:945449/1‑69 (MQ=255)aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:735796/1‑69 (MQ=255)aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:2695186/1‑69 (MQ=255)aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:3083280/1‑69 (MQ=255)aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGAc > 1:1794214/1‑69 (MQ=255) ggATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACAACGAAAGACtt > 1:246290/2‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGaaa > 1:1734312/1‑64 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:563778/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:1674711/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:3197476/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:3101852/1‑69 (MQ=255) cGATAGTCGCGATGGTCACCAGTGTCATAAAGAAGACGCCGGTTGCCCCACGGTTACCACGAAAGACtt > 1:692577/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:3224017/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:596511/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:2664932/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:2505229/1‑69 (MQ=255) tAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTAt > 1:1043853/1‑69 (MQ=255) aTAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga > 1:1386960/1‑69 (MQ=255) aTAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga > 1:2025565/1‑69 (MQ=255) aTAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga > 1:259817/1‑69 (MQ=255) aaaGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt < 1:978088/69‑1 (MQ=255) aaGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt < 1:747628/68‑1 (MQ=255) aaGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt < 1:1313634/68‑1 (MQ=255) cGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGaa < 1:2284370/69‑1 (MQ=255) cGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGaa < 1:2620254/69‑1 (MQ=255) cGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGaa < 1:757020/69‑1 (MQ=255) cGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGaa < 1:1611571/69‑1 (MQ=255) gCCGGTTGCCCCACTGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAAt < 1:1207139/69‑1 (MQ=255) gCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAAt < 1:462235/69‑1 (MQ=255) ttGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTg < 1:848708/69‑1 (MQ=255) tGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTg < 1:2814948/68‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:1137752/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:240186/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:622767/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:1385592/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:155444/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:3353657/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:1952052/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:3139732/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:3125091/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:1957558/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:2939751/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:2760298/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:2225549/69‑1 (MQ=255) aCGGTTACCACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:2539806/69‑1 (MQ=255) aCGGTTACAACGAAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTc < 1:715232/69‑1 (MQ=255) gTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCa > 1:311686/1‑67 (MQ=255) gTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCa > 1:215131/1‑67 (MQ=255) gTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCa > 1:125471/1‑67 (MQ=255) gTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCa > 1:794664/1‑67 (MQ=255) gTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCa > 1:2253333/1‑67 (MQ=255) gTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCa > 1:966468/1‑67 (MQ=255) | AACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCA > minE/1478913‑1479033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |