| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,479,278 | A→G | 18.0% | L217L (TTG→CTG) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,479,278 | 0 | A | G | 18.0% | 199.8 / 29.0 | 127 | L217L (TTG→CTG) | glpT | sn‑glycerol‑3‑phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base A (40/64); new base G (9/14); total (49/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CCTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGG > minE/1479215‑1479337 | ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1263616/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1795911/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1646439/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:188858/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:2064251/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1405597/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:2263001/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1024602/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:2580225/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:3090455/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1132082/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:953807/66‑1 (MQ=255)ccTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:992885/66‑1 (MQ=255) ccGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGAtt > 1:21130/1‑69 (MQ=255) ccGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGAtt > 1:991690/1‑69 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAGGCCACAGGATTGCg < 1:3043204/69‑1 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAGGCCACAGGATTGCg < 1:3038934/69‑1 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAGGCCACAGGATTGCg < 1:2416783/69‑1 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAGGCCACAGGATTGCg < 1:331372/69‑1 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAGGCCACAGGATTGCg < 1:841325/69‑1 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAGGCCACAGGATTGCg < 1:1871444/69‑1 (MQ=255) cTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGAACGGCGGCAGGCCACAGGATTGCg < 1:784619/69‑1 (MQ=255) ttttCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1975585/55‑1 (MQ=255) tCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGt < 1:2321536/68‑1 (MQ=255) tCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGt < 1:2221588/68‑1 (MQ=255) tCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGt < 1:1104965/68‑1 (MQ=255) tCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGt < 1:1293010/68‑1 (MQ=255) cGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGt < 1:2933921/67‑1 (MQ=255) ataGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACATGATTGCGGGGTATcgc > 1:1886716/1‑68 (MQ=255) ataGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATcgcg > 1:413992/1‑69 (MQ=255) ataGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATcgcg > 1:634854/1‑69 (MQ=255) ataGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATcgcg > 1:655457/1‑69 (MQ=255) tcgtcCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGc < 1:1723965/43‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:307417/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:3288673/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:1162795/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:354344/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:2838435/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:2641553/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:744766/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:75455/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:1783492/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:1576390/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAg < 1:193795/39‑1 (MQ=255) tAATCATTTTTGTACTCTTCGATCGGAGGCAAGCCACAg < 1:1195023/39‑1 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:1746360/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:2670339/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:2635667/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:121390/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:2574857/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:631983/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:859517/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:2282229/1‑68 (MQ=255) cATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCg > 1:1390033/1‑68 (MQ=255) aTTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCg > 1:1415254/1‑41 (MQ=255) aTTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGa > 1:1531207/1‑68 (MQ=255) aTTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGa > 1:569628/1‑68 (MQ=255) aTTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGa > 1:2256328/1‑68 (MQ=255) aTTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGa > 1:474284/1‑68 (MQ=255) ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat < 1:823478/68‑1 (MQ=255) ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat < 1:2136137/68‑1 (MQ=255) ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat < 1:1796094/68‑1 (MQ=255) ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat < 1:1059976/68‑1 (MQ=255) ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat < 1:195644/68‑1 (MQ=255) aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc < 1:2055613/68‑1 (MQ=255) aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc < 1:2870282/68‑1 (MQ=255) aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc < 1:599254/68‑1 (MQ=255) aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc < 1:234171/68‑1 (MQ=255) aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc < 1:778059/68‑1 (MQ=255) aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCACATCATCGCAAAGGCGAACAGTGcc < 1:1786919/68‑1 (MQ=255) ctctTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGccac < 1:1040397/69‑1 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGcaacca > 1:1742687/1‑68 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:2411896/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:1706251/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:1574957/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:1568036/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:2660483/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:469677/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:454258/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg > 1:3058333/1‑69 (MQ=255) ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCAACCAg > 1:54233/1‑69 (MQ=255) tCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat < 1:1347365/57‑1 (MQ=255) cGATCGGCGTCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGTCGAATAATGCCACCAg < 1:1655617/67‑1 (MQ=255) cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCTACCAGaa > 1:1094397/1‑69 (MQ=255) cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa > 1:860509/1‑69 (MQ=255) cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa > 1:2498881/1‑69 (MQ=255) cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa > 1:18196/1‑69 (MQ=255) cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa > 1:2094962/1‑69 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGTGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:2926525/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:143953/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:238854/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:3173236/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:1190398/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:523000/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:1194760/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:2677165/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:1072109/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:1725432/67‑1 (MQ=255) cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg < 1:3125886/67‑1 (MQ=255) cGATAGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa > 1:3172906/1‑69 (MQ=255) | CCTGTTCCGCTTTTTCGTTATAGTCGTCCGGATAATCATTTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGG > minE/1479215‑1479337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |