Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,479,321 T→C 17.8% L202L (TTA→TTG) ‡ glpT ← sn‑glycerol‑3‑phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,479,3210TC17.8% 147.1 / 20.3 95L202L (TTA→TTG) ‡glpTsn‑glycerol‑3‑phosphate transporter
Reads supporting (aligned to +/- strand):  ref base T (29/49);  new base C (9/8);  total (38/57)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 104 total aligned reads displayed.

TTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTC  >  minE/1479254‑1479374
                                                                   |                                                     
ttttGTACTCTTCGATCGGCGGCAAGCGACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:3192733/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:1219597/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:195644/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:1472222/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:2136137/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:1796094/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:823478/68‑1 (MQ=255)
ttttGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:1059976/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:599254/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:2870282/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:234171/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:2278222/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:778059/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:2055613/68‑1 (MQ=255)
      aCTCTTCGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCACATCATCGCAAAGGCGAACAGTGcc                                                 <  1:1786919/68‑1 (MQ=255)
       ctctTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGccac                                               <  1:1040397/69‑1 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGcaacca                                             >  1:1742687/1‑68 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:1711667/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:1706251/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:2411896/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:189829/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:2656802/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:2660483/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:3058333/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:454258/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:469677/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:631791/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:1574957/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            >  1:1568036/1‑69 (MQ=255)
          ttCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCAACCAg                                            >  1:54233/1‑69 (MQ=255)
           tCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGaat                                                       <  1:1347365/57‑1 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCTACCAGaa                                          >  1:1094397/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:492884/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:18196/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:2498881/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:2094962/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:860509/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:2352197/1‑69 (MQ=255)
            cGATCGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:1092813/1‑69 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGTGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:2926525/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:366430/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:1190398/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:143953/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:1186530/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:523000/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:2677165/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:68232/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:1072109/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:888697/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:1194760/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:3125886/67‑1 (MQ=255)
            cGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAg                                            <  1:1725432/67‑1 (MQ=255)
            cGATAGGCGGCAGGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGCCACCAGaa                                          >  1:3172906/1‑69 (MQ=255)
                cggcggCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATgg                                       >  1:1760204/1‑68 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCata                      <  1:2954193/69‑1 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCata                      <  1:1843071/69‑1 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCata                      <  1:1178618/69‑1 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCata                      <  1:2642293/69‑1 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCata                      <  1:281854/69‑1 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATCGCGCAGAAAGCATGCata                      <  1:1434236/69‑1 (MQ=255)
                                gATTGCGGGGTATCGCGCATCATCGCAAAGGCGAACAGTGcc                                                 <  1:1660984/42‑1 (MQ=38)
                                 aTTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCata                      <  1:57650/68‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:3218551/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:2858138/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:311006/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:2187086/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:2911245/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:2110634/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:833939/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:3030888/69‑1 (MQ=255)
                                   tGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:1824296/69‑1 (MQ=255)
                                       gggTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATag                   <  1:269093/65‑1 (MQ=255)
                                            tCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGcgccgc           >  1:1623817/1‑68 (MQ=255)
                                            tCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCa          >  1:2455122/1‑69 (MQ=255)
                                            tCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCa          >  1:3425515/1‑69 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCa                        >  1:937571/1‑53 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCa                        <  1:989879/53‑1 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCa                        <  1:502331/53‑1 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCa                        <  1:3315503/53‑1 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCa                        <  1:2840326/53‑1 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATagag                 >  1:1938050/1‑60 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGcgccg            >  1:1427617/1‑65 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCa          >  1:1853906/1‑67 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCa          >  1:548750/1‑67 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCAt         >  1:2893523/1‑68 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCAt         >  1:1954507/1‑68 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCAt         >  1:73561/1‑68 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCAt         >  1:692376/1‑68 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCAt         >  1:1455785/1‑68 (MQ=255)
                                              gcgcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCAt         >  1:1316355/1‑68 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:2529684/69‑1 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:2583092/69‑1 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:145790/69‑1 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:2821353/69‑1 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:2834929/69‑1 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:1613417/69‑1 (MQ=255)
                                                   tcatcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:1664424/69‑1 (MQ=255)
                                                    catcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGt   <  1:1314712/68‑1 (MQ=255)
                                                    catcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTc  <  1:1121087/69‑1 (MQ=255)
                                                    catcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTc  <  1:1548812/69‑1 (MQ=255)
                                                                   |                                                     
TTTTGTACTCTTCGATCGGCGGCAAGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTC  >  minE/1479254‑1479374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑