| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,480,246 | C→T | 40.5% | G16G (GGC→GGT) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,480,246 | 0 | C | T | 40.5% | 29.4 / 39.8 | 64 | G16G (GGC→GGT) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (28/10); new base T (16/10); total (44/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGC > minE/1480179‑1480313 | cGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggct > 1:1587845/1‑68 (MQ=255)cGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggcg > 1:2838512/1‑69 (MQ=255)cGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggcg > 1:2509464/1‑69 (MQ=255)cGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggcg > 1:1770474/1‑69 (MQ=255)cGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggcg > 1:2273823/1‑69 (MQ=255)cGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTggcggcg > 1:702018/1‑69 (MQ=255) ggATAACAATGAAAACTCGCGACTCTCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAg > 1:3355764/1‑69 (MQ=255) ggATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAg > 1:895027/1‑69 (MQ=255) ggATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAg > 1:260176/1‑69 (MQ=255) aCAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCggg > 1:1431792/1‑69 (MQ=255) aCAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg > 1:1773981/1‑68 (MQ=255) aCAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg > 1:752899/1‑68 (MQ=255) aCAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg > 1:2269627/1‑68 (MQ=255) aCAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCg > 1:2671398/1‑67 (MQ=255) aCAATCAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg < 1:2544358/68‑1 (MQ=255) aCAATCAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg < 1:1153049/68‑1 (MQ=255) aCAATCAAAACCCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg < 1:3263568/68‑1 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCggg > 1:2876083/1‑68 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:277710/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:1819857/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:747877/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:2090430/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:3153620/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:1635610/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:3222703/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:1415459/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:1392281/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:2571564/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:756595/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:3335924/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:2390224/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:2484132/1‑69 (MQ=255) cAATGAAAACTCGCGACTCGCAATCAAGAGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGa > 1:484015/1‑69 (MQ=255) aaTGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGTGAGCCGGGa > 1:671671/1‑68 (MQ=255) aaTGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGAt > 1:2881839/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:3328957/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:1806525/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:2803119/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:1810921/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:3018417/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:1558514/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:2644857/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:3061738/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:2296977/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:1046944/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:2348412/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc > 1:3150354/1‑69 (MQ=255) aaaaCTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGc > 1:2943795/1‑68 (MQ=255) gcgACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGAc < 1:1770821/68‑1 (MQ=255) gACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg < 1:847725/53‑1 (MQ=255) gACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGcc < 1:244818/60‑1 (MQ=255) gACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGACgg < 1:2731633/53‑1 (MQ=255) aCTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCgg < 1:2177387/52‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:1559379/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:2492333/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:1662472/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:2755077/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:2674552/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:2152903/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:2347488/59‑1 (MQ=255) tCAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:59892/59‑1 (MQ=255) cAAGTGACGTGATTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:1093270/58‑1 (MQ=255) gATTATCATTGGCGGCGGCGCAACGTGAGCCGGGATAGCCCGCGACtgtg > 1:2117212/1‑50 (MQ=39) gATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt > 1:557883/1‑69 (MQ=255) gATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt > 1:733874/1‑69 (MQ=255) gATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGt > 1:1195372/1‑69 (MQ=255) aTTATCATTGGCGGTGGCGCAACGGGAGCCGGGATTGCCCGCGACtg < 1:1261886/47‑1 (MQ=255) aTTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTg > 1:863736/1‑69 (MQ=255) aTTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTg < 1:2041198/69‑1 (MQ=255) ttGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTg < 1:2520318/68‑1 (MQ=255) gcggcgCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGTGGGCTGCGCGTGATTTTGGTTGAgc < 1:1445026/69‑1 (MQ=255) gcggcgCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAgc < 1:417972/69‑1 (MQ=255) gcggcgCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAgc < 1:2543925/69‑1 (MQ=255) gcggcgCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAgc < 1:117274/69‑1 (MQ=255) | CGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTGTGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGC > minE/1480179‑1480313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |