| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,480,522 | T→C | 56.7% | I108I (ATT→ATC) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,480,522 | 0 | T | C | 56.7% | 13.2 / 38.1 | 53 | I108I (ATT→ATC) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (5/18); new base C (19/11); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGA > minE/1480470‑1480587 | gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGaagaag < 1:1301710/69‑1 (MQ=255)gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGaagaag < 1:2710896/69‑1 (MQ=255)gCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGaagaag < 1:2261670/69‑1 (MQ=255) tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAggg < 1:3056230/67‑1 (MQ=255) tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAggg < 1:3005215/67‑1 (MQ=255) tATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAggg < 1:2823377/67‑1 (MQ=255) cACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg > 1:933748/1‑69 (MQ=255) cACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg > 1:2243984/1‑69 (MQ=255) cACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg > 1:1551428/1‑69 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCt < 1:1632214/69‑1 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:2804656/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:828088/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:2729872/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:1461251/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:2579502/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:2324507/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:3124961/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:2126830/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:427199/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAACGCAGAAGcc < 1:398817/69‑2 (MQ=255) gAAGATGACCTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCAAAGCAGAAGcc < 1:100225/69‑2 (MQ=255) aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta < 1:357411/69‑1 (MQ=255) aaGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta < 1:1921632/69‑1 (MQ=255) aGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtat < 1:2222678/69‑1 (MQ=255) aGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtat < 1:2026802/69‑1 (MQ=255) aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCt < 1:678158/62‑1 (MQ=255) aCCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGAcc > 1:1910987/1‑69 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:3426566/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:3359883/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:3265351/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1010002/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:2936498/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:722697/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1287354/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1333594/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:2218497/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1442206/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1752243/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1813205/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1849966/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:214556/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:2208037/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1419694/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:1342291/1‑50 (MQ=255) ccTCTCCTTCCAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:2624157/1‑50 (MQ=255) ccTCTCCTTACAGGCCACTTTTATCCGCGCCTGCGAAGAAGCAGGGATCa > 1:2736136/1‑50 (MQ=255) tctcCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgc < 1:2238959/69‑1 (MQ=255) ggCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg < 1:2272147/69‑1 (MQ=255) aCTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAtt < 1:1141533/69‑1 (MQ=255) cTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCAtt < 1:214651/68‑1 (MQ=255) tttATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTAt > 1:50964/1‑68 (MQ=255) aTTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGa < 1:3198485/68‑1 (MQ=255) aTTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGa < 1:2834742/68‑1 (MQ=255) | GCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGA > minE/1480470‑1480587 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |