| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,480,660 | C→A | 32.6% | T154T (ACC→ACA) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,480,660 | 0 | C | A | 32.6% | 118.9 / 62.6 | 101 | T154T (ACC→ACA) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (44/24); new base A (22/11); total (66/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
AACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGT > minE/1480598‑1480719 | aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:1825332/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:75323/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:65725/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:2431246/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:2436225/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:2482852/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:2578178/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:3121854/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagca > 1:2947732/1‑69 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagc > 1:1019872/1‑68 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagc > 1:954468/1‑68 (MQ=255)aaCCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCgcagc > 1:580432/1‑68 (MQ=255) ccGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCaaa < 1:3107366/68‑1 (MQ=255) ccGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCaaa < 1:2384144/68‑1 (MQ=255) ccGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCaaa < 1:402815/68‑1 (MQ=255) cTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:2868227/1‑69 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCAGCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:51905/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:97680/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:289075/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:3275452/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:2473452/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:433569/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:85428/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:948087/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:1909391/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:1873063/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:106398/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:1588028/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:1686285/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:1386089/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:1731989/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:1832474/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg < 1:1834524/67‑1 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTg > 1:1071177/1‑67 (MQ=255) gATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGAAGCAAACATGCTg > 1:3299913/1‑67 (MQ=255) gAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCt > 1:86077/1‑54 (MQ=255) gAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAaca > 1:3051801/1‑69 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:2160977/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:1513728/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:314940/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:3164112/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:3339882/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:3363346/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:351624/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:284406/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:2784266/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:2681482/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:1441967/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:455587/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:2555983/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:545250/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:633/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:64138/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:1950544/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:1202503/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:767542/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgca > 1:2183003/1‑39 (MQ=255) gTTCCGGATGGCACCGTTGATCCATTTCGTCTGACAgc > 1:1973988/1‑38 (MQ=255) gTTCCGGATGGCACCGTTGAGCCATTTCGTCTGACAgca > 1:805232/1‑39 (MQ=37) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAAGATGCTGGATGCCAAAGAACACGGTGCCGt < 1:513442/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGtt < 1:1016727/69‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:1178138/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:2953125/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:561504/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:1688052/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:1416238/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:2502567/68‑1 (MQ=255) gATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGt < 1:2906264/68‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:3108442/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:1855097/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:851903/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:1480257/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:364825/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:344111/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:3095893/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:3134633/54‑1 (MQ=255) gTTGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:1500091/54‑1 (MQ=255) gTTGATCCATTACGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:1193799/54‑1 (MQ=255) ttGATCCATTTCGTCTGACAGCAGCAAACATGCTGGATGCCAAAGAACACGGt < 1:1861494/53‑1 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTTGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:2463633/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGc > 1:167985/1‑68 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:3274217/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:1885961/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:842296/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:681913/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:2633421/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCt > 1:802196/1‑69 (MQ=255) gATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACAGCt > 1:1625610/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAg < 1:1647498/35‑1 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAg < 1:3102497/35‑1 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAg < 1:221741/35‑1 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAg < 1:2372049/35‑1 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:220068/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:420838/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:1771315/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:1912396/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:2467129/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:1619823/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGt > 1:2913118/1‑69 (MQ=255) tCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTAATGAAGt > 1:774624/1‑69 (MQ=255) | AACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAACATGCTGGATGCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGT > minE/1480598‑1480719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |